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Tissue Engineering der Harnblase

Tissue engineering of the urinary bladder

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Zusammenfassung

Im Tissue Engineering der Harnblase mit autologer Zelltransplantation ist die hochgradige Differenzierung der in vitro auf biokompatiblen Matrizes kultivierten Zellen für die Funktionalität der Gewebekonstrukte nach der Implantation essenziell. In diesem Zusammenhang hat die terminale Differenzierung der oberflächlichen Urothelzellen (UZ) aufgrund deren Barrierefunktion gegen Urin eine entscheidende Rolle. In dieser Arbeit beschäftigen wir uns mit der Bestimmung von optimierten Konditionen für die Bildung von terminal differenziertem Urothel für die Beschichtung von größeren Flächen biologischer Membranen. Das kann uns dem Ziel der klinischen Anwendung von funktionierenden Gewebekonstrukten näher bringen.

Abstract

In tissue engineering of the urinary bladder with autologous cell transplantation, high differentiation of the cells cultivated in vitro on biocompatible membranes is essential for the functionality of the tissue constructs after implantation. The terminal differentiation of superficial urothelial cells has a key role because of the barrier function of these cells against urine. The aim of this study was to determine optimized conditions for the creation of terminally differentiated urothelium to cover large membrane surfaces. This can bring us closer to the goal of using functioning tissue constructs in clinical trials.

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Danksagung

Die Autoren danken Frau Dr. Uta Schmidt für die kritische Durchsicht des Manuskripts.

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Der korrespondierende Autor versichert, dass keine Verbindungen mit einer Firma, deren Produkt in dem Artikel genannt ist, oder einer Firma, die ein Konkurrenzprodukt vertreibt, bestehen.

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Authors and Affiliations

  1. Klinik und Poliklinik für Urologie, Technische Universität , Dresden

    G. Ram-Liebig, O. W. Hakenberg & M. P. Wirth

  2. Klinik und Poliklinik für Urologie, Technische Universität , Fetscherstraße 74, 01307 , Dresden

    G. Ram-Liebig

Authors
  1. G. Ram-Liebig
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  2. O. W. Hakenberg
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  3. M. P. Wirth
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Correspondence to G. Ram-Liebig.

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Ram-Liebig, G., Hakenberg, O.W. & Wirth, M.P. Tissue Engineering der Harnblase. Urologe [A] 43, 1217–1222 (2004). https://doi.org/10.1007/s00120-004-0690-7

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  • DOI: https://doi.org/10.1007/s00120-004-0690-7

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