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Fast differentiation of Apocynum venetum with related species by UPLC/MS and UPLC/SPE/NMR

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Abstract

The newly established hyphenated instrumentation of UPLC/MS and UPLC/SPE/NMR techniques has been applied for a fast comparison of the major chemical components from Chinese medicinal herb Apocynum venetum and related species. The content of the compounds among three species was found to be highly variable qualitatively as well as quantitatively. The identification of the constituents was based on matching their UV spectrum, online UPLC/MS/MS, and UPLC/SPE/NMR data. The system successfully identified 16 compounds in one step via operation for 4 h. Hyperforin was described for the first time in A. venetum, all of which had not previously been reported in Apocynum hendersonii and Apocynum pictum.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (No.30970299), and Major Project of Jilin Provincial Science and Technology Department (No.20090936).

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Authors and Affiliations

  1. Central Laboratory, Changchun Normal University, Changchun, 130032, China

    Yuchi Zhang, Chunming Liu, Yanjuan Qi, Guimei Wu & Sainan Li

  2. Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling, 136100, China

    Zhengkun Zhang

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  1. Yuchi Zhang
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  2. Chunming Liu
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  3. Zhengkun Zhang
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  4. Yanjuan Qi
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  5. Guimei Wu
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  6. Sainan Li
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Correspondence to Chunming Liu.

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Zhang, Y., Liu, C., Zhang, Z. et al. Fast differentiation of Apocynum venetum with related species by UPLC/MS and UPLC/SPE/NMR. Med Chem Res 21, 1077–1083 (2012). https://doi.org/10.1007/s00044-011-9624-2

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  • DOI: https://doi.org/10.1007/s00044-011-9624-2

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