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Role of vasostatin-1 C-terminal region in fibroblast cell adhesion

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Abstract

Fibroblast adhesion can be modulated by proteins released by neuroendocrine cells and neurons, such as chromogranin A (CgA) and its N-terminal fragment vasostatin-1 (VS-1, CgA1–78). We have investigated the mechanisms of the interaction of VS-1 with fibroblasts and of its pro-adhesive activity and have found that the proadhesive activity of VS-1 relies on its interaction with the fibroblast membrane via a phospholipid-binding amphipathic α-helix located within residues 47–66, as well as on the interaction of the adjacent C-terminal region 67–78, which is structurally similar to ezrin–radixin–moesin-binding phosphoprotein 50 (a membrane-cytoskeleton adapter protein), with other cellular components critical for the regulation of cell cytoskeleton.

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Abbreviations

CgA:

Chromogranin A

VS-1:

Recombinant Ser-Thr-Ala-CgA1–78

mAb:

Monoclonal antibody

ERM:

Ezrin–radixin–moesin

EBP50:

ERM-binding phosphoprotein 50

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Acknowledgments

This work was supported by Associazione Italiana per la Ricerca sul Cancro (AIRC) and Ministero della Sanità of Italy. We thank Alessandra Boletta for helpful discussion.

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Authors and Affiliations

  1. Division of Molecular Oncology and IIT Network Research Unit of Molecular Neuroscience, San Raffaele Scientific Institute, via Olgettina 58, 20132, Milan, Italy

    Eleonora Dondossola, Anna Gasparri, Angela Bachi, Flavio Curnis & Angelo Corti

  2. Consiglio Nazionale delle Ricerche, Milan, Italy

    Renato Longhi

  3. INSERM U575, Strasbourg, France

    Marie-Hélène Metz-Boutigue

  4. Department of Cell Biology, University of Calabria, Cosenza, Italy

    Bruno Tota

  5. Department of Biomedicine, University of Bergen, Bergen, Norway

    Karen B. Helle

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  1. Eleonora Dondossola
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  2. Anna Gasparri
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  3. Angela Bachi
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  4. Renato Longhi
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  5. Marie-Hélène Metz-Boutigue
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  6. Bruno Tota
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  7. Karen B. Helle
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  8. Flavio Curnis
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  9. Angelo Corti
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Correspondence to Angelo Corti.

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Dondossola, E., Gasparri, A., Bachi, A. et al. Role of vasostatin-1 C-terminal region in fibroblast cell adhesion. Cell. Mol. Life Sci. 67, 2107–2118 (2010). https://doi.org/10.1007/s00018-010-0319-5

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  • DOI: https://doi.org/10.1007/s00018-010-0319-5

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