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Purification and analysis of DNA from low DNA content food samples

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Abstract

Recently, molecular biology techniques are widely used in food analysis; yet, the complexity of food matrices causes difficulty in purification and analysis of the analyte. The aim of this study was to introduce a new material, zeolite Y, that is able to isolate DNA even from low DNA content food samples; and, to demonstrate the integrity of isolated DNA via real-time polymerase chain reaction. Although the purity of the isolated DNA has to be improved with further work, Zeolite Y was shown to have a potential to be effectively used in DNA isolation for food analysis especially for low DNA content food samples.

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Acknowledgements

This research is supported by the Project No. 115O065 of TUBITAK TOVAG 1003.

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Authors and Affiliations

  1. TUBITAK MRC, Food Institute, P.O. Box 21, 41470, Gebze, Kocaeli, Turkey

    M. Esra Altuntop Yayla & Canan Ekinci Doğan

  2. TUBITAK MRC, Chemistry Institute, P.O. Box 21, 41470, Gebze, Kocaeli, Turkey

    Şerife Sarioğlan

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  1. M. Esra Altuntop Yayla
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  2. Canan Ekinci Doğan
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  3. Şerife Sarioğlan
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Correspondence to M. Esra Altuntop Yayla or Canan Ekinci Doğan.

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Altuntop Yayla, M.E., Ekinci Doğan, C. & Sarioğlan, Ş. Purification and analysis of DNA from low DNA content food samples. J Consum Prot Food Saf 14, 147–155 (2019). https://doi.org/10.1007/s00003-018-1192-7

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  • DOI: https://doi.org/10.1007/s00003-018-1192-7

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