Abstract:
Objective and design: The consequences of Kupffer cell phagocytosis blockade were studied in endotoxemic rats with obstructive jaundice.¶Material or Subjects: 159 male Wistar rats.¶Treatment: Obstructive jaundice was induced by bile duct ligation (BDL). Gadolinium chloride (1 mg/100g iv) was given 6 days after BDL to inhibit Kupffer cell activity and the animals were challenged with 1 mg/g endotoxin 24 h later.¶Methods: Endotoxin sensitivity, tumor necrosis factor-α and interleukin-6 production were studied, liver and lung injury were assessed by neutrophil infiltration assay, tissue adenosine triphosphate, aspartate aminotransferase, alanine aminotransferase level determinations and histology, respectively. For statistics non-parametric methods were used.¶Results: BDL sensitized the animals to endotoxin, increased endotoxin-induced tumor necrosis factor-α and interleukin-6 production and reduced ATP contents of the liver and the lung. Kupffer cell blockade significantly increased the resistance against endotoxin, diminished the inflammatory cytokine release and reduced endotoxin-induced tissue injury in BDL animals.¶Conclusion: Attenuation of Kupffer cell function decreases endotoxin-induced lethality and morbidity in obstructive jaundice.
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Received 3 January 2002; returned for revision 24 May 2002; accepted by E. Neugebauer 8 July 2002
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ID="*"Correspondence to: G. Lázár Jr.
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Lázár Jr., G., Paszt, A., Kaszaki, J. et al. Kupffer cell phagocytosis blockade decreases morbidity in endotoxemic rats with obstructive jaundice. Inflamm. res. 51, 511–518 (2002). https://doi.org/10.1007/PL00012421
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DOI: https://doi.org/10.1007/PL00012421