Skip to main content
SpringerLink
Log in

Simultaneous radioimmunoassay for plasma arginine-vasopressin and oxytocin using DEAE Sephadex A 25 extraction

  • Published:
Journal of Endocrinological Investigation Aims and scope Submit manuscript

Abstract

A sensitive and specific radioimmunoassay method for simultaneous measurement of plasma arginine vasopressin (AVP) and oxytocin (OT) has been developed utilizing an extraction technique on DEAE Sephadex A 25. This procedure resulted in mean recoveries of 70.7% (AVP) and 65.4% (OT) in the peptide range of 5 to 100 pg/4 ml. The sensitivity of the assay is 0.5 pg/tube for AVP and 2 pg/tube for OT. The lower limit of detection for plasma extracts was 1.2 pg AVP/ml and 5 pg OT/ml plasma. Employing this method in normal human non smokers and ad libitum fluid the basal levels (mean ± SE) of plasma AVP are 3.5 ± 0.2 pg/ml in males and 4.6 ± 0.4 pg/ml in females and the basal concentrations of plasma OT are 5.1 ± 0.3 pg/ml in males and 5.4 ± 0.3 pg/ml in females. Dehydration and water loading produced significant changes in plasma AVP and OT concentrations and a significant correlation exists between plasma AVP and plasma (r= 0.96, p < 0.001 ) and urinary (r = 0.84, p < 0.01 ) osmolality, but not between plasma OT concentrations and plasma (r =0.11, NS) and urinary (r = 0.27, NS) osmolality. These results suggest that a wide range of physiological and pathophysiological changes in plasma AVP and OT can be simultaneously measured by the extraction procedure and the radioimmunoassay described.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Robertson G.L., Klein L.A., Roth J., Gorden P. Immunoassay of plasma vasopressin in man. Proc. Natl. Acad. Sci USA 66: 1298, 1970.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  2. Beardwell C.G. Radioimmunoassay of arginine vasopressin in normal plasma. J. Clin. Endocrinol. Metab. 33: 254, 1971.

    Article  CAS  PubMed  Google Scholar 

  3. Robertson G.L., Mahr E.A., Athar S., Sinha T. Development and clinical application of a new method for the radioimmunoassay of arginine vasopressin in human plasma. J. Clin. Invest. 52: 2340, 1973.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. Husain M.K., Fernando N., Shapiro M., Kagan A., Glick S.M. Radioimmunoassay of arginine vasopressin in human plasma. J. Clin. Endocrinol. Metab. 37: 616, 1973.

    Article  CAS  PubMed  Google Scholar 

  5. Rosembloom A.A., Sack J., Fisher D.A. The circulation vasopressinase of pregnancy: species comparison with radioimmunoassay. Am. J. Obstet. Gynecol. 121: 316, 1975.

    Google Scholar 

  6. Skowsky W.R., Fisher D.A. The use of thyroglobulin to induce antigenicity to small molecule. J. Lab. Clin. Med. 80: 134, 1972.

    CAS  PubMed  Google Scholar 

  7. Permutt M.A., Parker C.W., Utiger R.D. Immunochemical studies with lysine vasopressin. Endocrinology 78: 809, 1966.

    Article  CAS  PubMed  Google Scholar 

  8. Legros J.J., Stewart U., Nordmann J.J., Dreifuss J.J., Franchimont P. Libération de Vasopressine, d’ocytocine e de neurophysine estimée per methode radio-immunologique lors de la stimulation in vitro de neurohypophyses de rat. C.R. Soc. Biol. 165: 2443, 1971.

    CAS  Google Scholar 

  9. Fyhrquist F., Wallenius M., Holleman H.J.G. Radioimmunoassay of vasopressin in unextracted plasma. Scand. J. Clin. Lab. Invest. 36: 841, 1976.

    Article  CAS  PubMed  Google Scholar 

  10. Hunter W.M., Greenwood F.C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature 194: 495, 1962.

    Article  CAS  PubMed  Google Scholar 

  11. Legros J.J., Franchimont P. The purification and modification of immunological and physicochemical behavior of iodinated posterior pituitary hormones. In: Kirkhan K.E., Hunter W.M. (Eds.), Radioimmunoassay Methods. Churcill-Livingstone, Edimburg, 1971, p. 40.

    Google Scholar 

  12. Desbuquois V., Aurbach G.D. Use of polyethylene glycol to separate free and antibody-bound peptide hormones in radioimmunoassay. J. Clin. Endocrinol. Metab. 33: 732, 1971.

    Article  CAS  PubMed  Google Scholar 

  13. Pullan P.T., Clappison B.H., Johnston C.I. Plasma vasopressin and human neorphysins in physiological and pathological states associated with changes in vasopressin secretion. J. Clin. Endocrinol. Metab. 49: 580, 1979.

    Article  CAS  PubMed  Google Scholar 

  14. Edwards C.R.W. Vasopressin and oxytocin in health and disease. In: Besser G.M. (Ed.), Clinics in Endocrinology and Metabolism. Saunders, London, 1977, vol. 6, p. 223.

    Article  CAS  PubMed  Google Scholar 

  15. Bartter F.C., Schwartz W.B. The syndrome of inappropriate secretion of antidiuretic hormone. Am. J. Med. 42: 790, 1967.

    Article  CAS  PubMed  Google Scholar 

  16. Morton J., Padfield P.L., Forsling M. A radioimmunoassay for plasma arginine-vasopressin in man and dog: application to physiological and pathological states. J. Endocrinol. 65: 411, 1975.

    Article  CAS  PubMed  Google Scholar 

  17. George C.P.L., Messerli F.H., Genest J., Nowaczynski W., Boucher R., Kuchel O., Rojo-Ortega M. Diurnal variation of plasma vasopressin in man. J. Clin. Endocrinol. Metab. 41: 332, 1975.

    Article  CAS  PubMed  Google Scholar 

  18. Miller P.D., Dobovsky S.L., Mac Donald K.M., Katz F.H., Robertson G.L., Schrier R.W. Centrai, renal and adrenal effects of lithium in man. Am. J. Med. 66: 797, 1979.

    Article  CAS  PubMed  Google Scholar 

  19. Thibonnier M., Soto M.E., Marchetti J., Corvol P., Menard J., Milliez P. Dosage radioimmunologique de la Vasopressine plasmatique. Ann. Endocrinol. (Paris) 41: 379, 1980.

    CAS  Google Scholar 

  20. Landgraf R. Simultaneous measurement of arginine vasopressin and oxytocin in plasma and neurohypophyses by radioimmunoassay. Endokrinologie 78: 191, 1981.

    CAS  PubMed  Google Scholar 

  21. Leake R.D., Weitzman R.E., Glatz T.N., Fisher D.A. Plasma oxytocin concentrations in men, non pregnant women and pregnant women before and during spontaneous labor. J. Clin. Endocrinol. Metab. 53: 730, 1981.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Psychoneuroendocrinology Section, Radioimmunoassay Laboratory and Department of Clinical and Medical Pathology, CHU, local 4/12, University of Liège, Sart Tilman, B-4000, Liège, Belgium

    F. Louis & J. J. Legros

  2. Istituto di Clínica Medica Generale e Terapia Medica, University of Parma, Via Gramsci 14, 43100, Parma, Italy

    Paolo Chiodera M.D.

Authors
  1. Paolo Chiodera M.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Louis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. J. Legros
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chiodera, P., Louis, F. & Legros, J.J. Simultaneous radioimmunoassay for plasma arginine-vasopressin and oxytocin using DEAE Sephadex A 25 extraction. J Endocrinol Invest 7, 287–293 (1984). https://doi.org/10.1007/BF03351004

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03351004

Key-words

Search

Navigation