Abstract
The response of circulating leukocytes to thyroid subcellular fractions was investigated in 19 patients with Graves’ disease, 15 patients with Hashimoto’s thyroiditis, 7 patients with toxic adenoma, 19 patients with nontoxic goiter and in 10 healthy students as control subjects. For this purpose, the leukocyte migration test of Soborg and Bendixen was performed against human crude thyroid extract (CTE), cell plasma membranes, nuclei, ribosomes, mitochondria and microsomes. Our results show positive LMT against: 1) CTE in patients with Graves’ disease (61 ± 13, p<0.001) and Hashimoto’s thyroiditis (65 ± 11, p<0.001) compared to controls (90 ± 11); 2) cell plasma membranes in patients with Graves’disease (41 ± 14, p<0.001) and Hashimoto’s thyroiditis (64 ± 21, p<0.05) compared to controls (88 ± 19); 3) nuclei in patients with Graves’ disease (53 ± 25, p<0.001) and Hashimoto’s thyroiditis (53 ± 23, p<0.001) compared to controls (83 ±11). Ourfindingsof circulating leukocytes sensitized to cell plasma membranes and nuclear fraction in patients with Graves’ disease and Hashimoto’s thyroiditis provide the additional information that these patients have a specific defect in immune-surveillance.
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Doniach D. Humoral and genetic aspects of thyroid autoimmunity. J. Clin. Endocrinol. Metab. 4: 267, 1975.
Doniach D., Vaughan Hudson R., Roitt I.M. Human autoimmune thyroiditis: clinical studies. Br. Med. J. 2: 365, 1960.
Doniach D., Nilsson L.R., Roitt I.M. Autoimmune thyroiditis in children and adolescents. Immunological correlations and parent study. Acta Paediatr. Scand. 54: 260, 1965.
Totterman T.H., Maenpaa J., Gordin A., Makinen T., Taskinen E., Andersson L.C., Hayry P. Blood and thyroid-infiltrating lymphocyte subclasses in juvenile-autoimmune thyroiditis. Clin. Exp. Immunol. 30: 193, 1977.
Farid N.R., Row V.V., Volpè R. Thyroid gland lymphocytes in Graves’ disease and Hashimoto’s thyroiditis. Clin. Res. 21: 1025, 1973.
Calder E.A., Mc Leman D., Barnes E.W., Irvine W.J. The effect of thyroid antigens on the in vitro-migration of leukocytes from patients with Hashimoto’s thyroiditis. Clin. Exp. Immunol. 12: 229, 1972.
Calder E.A., Irvine W.J. Cell-mediated immunity and immune-complexes in thyroid disease. Clin. Endocrinol. Metab. 4: 287, 1975.
Totterman T.M., T., Gordin A. Cell-mediated autoimmunity in thyroid disease as studied by the leukocyte migration test: correlation to clinical state and effect of treatment. Acta Endocrinol. (Kbh.) 86: 89, 1977.
Matsui Y., Beall G.N., Chopra J., Solomon D.H., Kruger S., Beall M. Assays for antigen-stimulated lymphocyte-de ed migration enhancement and inhibition factors from patients with thyroid diseases and their relatives. Acta Endocrinol. (Kbh.) 86: 732, 1977.
Delespesse G., Duchateau J., Collet H., Govaerts A., Bastenie P.A. Lymphocyte transformation with thyroglobulin in thyroid diseases. Clin. Exp. Immunol. 12: 439, 1972.
Galluzzo A., Giordano C., Andronico F., Filardo C., Andronico G., Bompiani G.D. Leukocyte migration test in subacute thyroiditis. Hypothetical role of cell-mediated immunity. J. Clin. Endocrinol. Metab. 50: 1038, 1980.
Soborg M., Bendixen G. Human lymphocyte migration as a parameter of hypersensivity. Acta Med. Scand. 181: 247, 1967.
Mäkinen T., Tötterman T.H., Gordin A., Weber T.H. Migration inhibition factor and the blood clotting system. Clin. Exp. Immunol. 29: 181, 1977.
Wartenberg J., Doniach D., Brostoff J., Roitt I.M. Leukocyte migration inhibition with mitochondria in human autoimmune thyroid disorders. Clin. Exp. Immunol. 14: 203, 1973.
Smith D.R., Hall R. Thyroid stimulating immunoglobulins in Graves’ disease. Lancet 2: 427, 1974.
Sugenoya A., Kidd A., Row V.V., Volpè R. Correlation between thyrotropin-displacing activity and human thyroid-stimulating activity by immunoglobulins from patients with Graves’ disease and other thyroid disorders. J. Clin. Endocrinol. Metab. 48: 398, 1979.
Sugenoya A., Troukodes K., Row V.V., Volpè R. The production of thyroid-stimulating immunoglobulins (TSI) by lymphocytes from patients with Graves’ disease cultured with human thyroid subcellular fractions. J. Endocrinol. Invest. 1: 245, 1978.
Mäkinen T., Wägar G., Apter L., Von Willebrand E., Pekonen F. Evidence that the TSH receptor acts as a mitogenic antigen in Graves’ disease. Nature 275: 314, 1978.
Volpè R. The role of autoimmunity in hypoendocrine and hyper-endocrine functions. Ann. Intern. Med. 87: 86, 1977.
Aoki N., Pinnamanemi K.M., De Groot L.J. Studies on suppressor cell function in thyroid disease. J. Clin. Endocrinol. Metab. 48: 803, 1979.
Galluzzo A., Giordano C., Sparacino G., Filardo C., Rubino G., Rubino R., Bompiani G.D. Cultura mista linfocitaria autologa in pazienti affetti da morbo di Graves. Valutazionedellafunzione suppressor. J. Endocrinol. Invest. 3 (Suppl. 1): 55, 1980.
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Galluzzo, A., Filardo, C., Giordano, C. et al. Leukocyte migration test (LMT) in patients with thyroid disease: the response to human thyroid subcellular fractions. J Endocrinol Invest 4, 173–176 (1981). https://doi.org/10.1007/BF03350447
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DOI: https://doi.org/10.1007/BF03350447