Abstract
Bone remodelling is regulated at the local level by an incompletely elucidated cytokine network. In the present study we have determined the effect of interleukin-4 (IL-4), a cytokine produced by T Iymphocytes and other cells, on the activity of murine osteoblasts. IL-4 (0.1–10 ng/ml) did not influence the proliferation rate of the osteoblast-like cell line MC3T3, but inhibited the expression of alkaline phosphatase. In long-term cultures supplemented with ascorbic acid and glycerophosphate such an effect was accompanied by a retardation of matrix mineralization. IL-4 also stimulated M-CSF expression by MC3T3 cells, both at the RNA and bioactivity levels. However, no stimulation of IL-1, IL-6, GM-CSF or PGE2 production was observed. An IL-4-induced inhibition of alkaline phosphatase expression and retardation of mineralization was also found in cultures of primary osteoblast-like cells isolated from neonatal mice calvariae. These results suggest that IL-4, probably released by cells within the bone marrow, may locally influence the activity of bone-forming cells.
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Riancho, J.A., González-Marcías, J., Amado, J.A. et al. Interleukin-4 as a bone regulatory factor: Effects on murine osteoblast-like cells. J Endocrinol Invest 18, 174–179 (1995). https://doi.org/10.1007/BF03347799
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DOI: https://doi.org/10.1007/BF03347799