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Effect of dietary glycemic index on food intake, adiposity, and fasting plasma ghrelin levels in animals

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Abstract

Background: An increase in lipid storage as a consequence of feeding animals with high-glycemic index (GI) diets has been observed by many authors. Ghrelin is one of the most important orexigenic hormones, and curiously, its fasting plasma levels are decreased in human obesity. Aim: As ghrelin secretion is affected by insulin concentration, we hypothesized that carbohydrates with different glycemic responses might influence fasting plasma ghrelin levels. Material and methods: Twenty rats were divided into two groups and fed adlibituma low-GI or a high-GI diet for 21 days. Results: In rats fed a high- vs low-GI diet we observed: increased food intake (18.9±0.6 vs 16.4±2.0 g/day; p<0.01), increased weight gain (28.8±6.6 vs 16.4±6% of initial weight; p<0.01), higher relative weight of epididymal fat pads (1.7±0.4 vs 1.4±0.3%; p=0.05), but lower total fasting ghrelin levels (41.1 ±10.7 vs 59.5±9.8 pg/ml; p=0.05). Conclusions: Ghrelin appeared to be downregulated in rats fed a high-GI diet; this observation could be related to the higher food intake and fat mass observed in these rats and to the effects of insulin response on ghrelin levels.

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References

  1. Livesey G, Taylor R, Hulshof T, Howlett J. Glycemic response and health—a systematic review and meta-analysis: relations between dietary glycemic properties and health outcomes. Am J Clin Nutr 2008, 87: 258S–68S.

    PubMed  CAS  Google Scholar 

  2. Howlett J, Ashwell M. Glycemic response and health: summary of a workshop. Am J Clin Nutr 2008, 87: 212S-6S.

    Google Scholar 

  3. Alfenas RC, Mattes RD. Influence of glycemic index/load on glycemic response, appetite, and food intake in healthy humans. Diabetes Care 2005, 28: 2123–9.

    Article  PubMed  Google Scholar 

  4. Flint A, Møller BK, Raben A, et al. Glycemic and insulinemic responses as determinants of appetite in humans. Am J Clin Nutr 2006, 84: 1365–73.

    PubMed  CAS  Google Scholar 

  5. Flint A, Gregersen NT, Gluud LL, et al. Associations between postprandial insulin and blood glucose responses, appetite sensations and energy intake in normal weight and overweight individuals: ameta-analysis of test meal studies. Br J Nutr 2007, 98: 17–25.

    Article  PubMed  CAS  Google Scholar 

  6. Thomas DE, Elliott EJ, Baur L. Low glycaemic index or low glycaemic load diets for overweight and obesity. Cochrane Database Syst Rev 2007, 18: CD005105.

  7. Kabir M, Rizkalla SW, Quignard-Boulangé A, et al. A high glycemic index starch diet affects lipid storage-related enzymes in normal and to a lesser extent in diabetic rats. J Nutr 1998, 128: 1878–83.

    PubMed  CAS  Google Scholar 

  8. Maegawa H, Kobayashi M, Ishibashi O, Takata Y, Shigeta Y. Effect of diet change on insulin action: difference between muscles and adipocytes. Am J Physiol 1986, 251: E616–23.

    PubMed  CAS  Google Scholar 

  9. Pawlak DB, Kushner JA, Ludwig DS. Effects of dietary glycaemic index on adiposity, glucose homoeostasis, and plasma lipids in animals. Lancet 2004, 364: 778–85.

    Article  PubMed  CAS  Google Scholar 

  10. Scribner KB, Pawlak DB, Aubin CM, Majzoub JA, Ludwig DS. Long-term effects of dietary glycemic index on adiposity, energy metabolism, and physical activity in mice. Am J Physiol Endocrinol Metab 2008, 295: E1126–31.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  11. McGarry JD, Mannaerts GP, Foster DW. A possible role for malonyl- CoA in the regulation of hepatic fatty acid oxidation and ketogenesis. J Clin Invest 1977, 60: 265–70.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  12. Ludwig DS. The glycemic index: physiological mechanisms relating to obesity, diabetes, and cardiovascular disease. JAMA 2002, 287: 2414–23.

    Article  PubMed  CAS  Google Scholar 

  13. Du H, van der A DL, van Bakel MM, et al. Glycemic index and glycemic load in relation to food and nutrient intake and metabolic risk factors in a Dutch population. Am J Clin Nutr 2008, 87: 655–61.

    PubMed  CAS  Google Scholar 

  14. Barclay AW, Petocz P, McMillan-Price J, et al. Glycemic index, glycemic load, and chronic disease risk, a meta-analysis of observational studies. Am J Clin Nutr 2008, 87: 627–37.

    PubMed  CAS  Google Scholar 

  15. De Vriese C, Delporte C. Influence of ghrelin on food intake and energy homeostasis. Curr Opin Clin Nutr Metab Care 2007, 10: 615–9.

    Article  PubMed  Google Scholar 

  16. Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature 1999, 402: 656–60.

    Article  PubMed  CAS  Google Scholar 

  17. Cummings DE, Purnell JQ, Frayo RS, Schmidova K, Wisse BE, Weigle DS. A preprandial rise in plasma ghrelin levels suggests a role in meal initiation in humans. Diabetes 2001, 50: 1714–9.

    Article  PubMed  CAS  Google Scholar 

  18. Callahan HS, Cummings D, Pepe M, Breen P, Matthys C, Weigle D. Postprandial suppression of plasma ghrelin level is proportional to ingested caloric load but does not predict intermeal interval in humans. J Clin Endocrinol Metab 2004, 89: 1319–24.

    Article  PubMed  CAS  Google Scholar 

  19. Tschöp M, Wawarta R, Riepl R, Bidlingmaier M, Landgraf R, Folwaczny C. Post-prandial decrease of circulating human ghrelin levels. J Endocrinol Invest 2001, 24: RC19–21.

    PubMed  Google Scholar 

  20. Tschöp M, Weyer C, Tataranni PA, Devanarayan V, Ravussin E, Heiman ML. Circulating ghrelin levels are decreased in human obesity. Diabetes 2001, 50: 707–9.

    Article  PubMed  Google Scholar 

  21. English PJ, Ghatei M, Malik I, Bloom S, Wilding J. Food fails to suppress ghrelin levels in obese humans. J Clin Endocrinol Metab 2002, 87: 2984–7.

    Article  PubMed  CAS  Google Scholar 

  22. McLaughlin T, Abbasi F, Lamendola C, Frayo RS, Cummings DE. Plasma ghrelin concentrations are decreased in insulin-resistant obese adults relative to equally obese insulin-sensitive controls. J Clin Endocrinol Metab 2004, 89: 1630–5.

    Article  PubMed  CAS  Google Scholar 

  23. Katsuki A, Urakawa H, Gabazza EC, et al. Circulating levels of active ghrelin is associated with abdominal adiposity, hyperinsulinemia and insulin resistance in patients with type 2 diabetes mellitus. Eur J Endocrinol 2004, 151: 573–7.

    Article  PubMed  CAS  Google Scholar 

  24. Dezaki K, Sone H, Yada T. Ghrelin is a physiological regulator of insulin release in pancreatic islets and glucose homeostasis. Pharmacol Ther 2008, 118: 239–49.

    Article  PubMed  CAS  Google Scholar 

  25. Pawlak DB, Bryson JM, Denyer GS, Brand-Miller JC. High glycemic index starch promotes hypersecretion of insulin and higher body fat in rats without affecting insulin sensitivity. J Nutr 2001, 131: 99–104.

    PubMed  CAS  Google Scholar 

  26. Scribner KB, Pawlak DB, Aubin CM, Majzoub JA, Ludwig DS. Long-term effects of dietary glycemic index on adiposity, energy metabolism, and physical activity in mice. Am J Physiol Endocrinol Metab 2008, 295: E1126–31.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  27. Pereira MA, Swain J, Goldfine AB, Rifai N, Ludwig DS. Effects of a low-glycemic load diet on resting energy expenditure and heart diseas risk factors during weight loss. JAMA 2004, 292: 2482–90.

    Article  PubMed  CAS  Google Scholar 

  28. Salbe AD, Tschöp MH, DelParigi A, Venti CA, Tataranni PA. Negative relationship between fasting plasma ghrelin concentrations and ad libitum food intake. J Clin Endocrinol Metab 2004, 89: 2951–6.

    Article  PubMed  CAS  Google Scholar 

  29. Nakagawa E, Nagaya N, Okumura H, et al. Hyperglycaemia suppresses the secretion of ghrelin, a novel growth-hormone-releasing peptide: responses to the intravenous and oral administration of glucose. Clin Sci (Lond) 2002, 103: 325–8.

    CAS  Google Scholar 

  30. Möhlig M, Spranger J, Otto B, Ristow M, Tschöp M, Pfeiffer AF. Euglycemic hyperinsulinemia, but not lipid infusion, decreases circulating ghrelin levels in humans. J Endocrinol Invest 2002, 25: RC36–8.

    Article  PubMed  Google Scholar 

  31. Greenman Y, Golani N, Gilad S, Yaron M, Limor R, Stern N. Ghrelin secretion is modulated in a nutrient- and gender-specific manner. Clin Endocrinol (Oxf) 2004, 60: 382–8.

    Article  CAS  Google Scholar 

  32. Broglio F, Arvat E, Benso A, et al. Ghrelin, a natural GH secretagogue produced by the stomach, induces hyperglycemia and reduces insulin secretion in humans. J Clin Endocrinol Metab 2001, 86: 5083–6.

    Article  PubMed  CAS  Google Scholar 

  33. Cui C, Ohnuma H, Daimon M, et al. Ghrelin infused into the portal vein inhibits glucose-stimulated insulin secretion in Wistar rats. Peptides 2008, 29: 1241–6.

    Article  PubMed  CAS  Google Scholar 

  34. Blom WA, Stafleu A, de Graaf C, Kok FJ, Schaafsma G, Hendriks HF. Ghrelin response to carbohydrate-enriched breakfast is related to insulin. Am J Clin Nutr 2005, 81: 367–75.

    PubMed  CAS  Google Scholar 

  35. Tschöp M, Weyer C, Tataranni PA, Devanarayan V, Ravussin E, Heiman ML. Circulating ghrelin levels are decreased in human obesity. Diabetes 2001, 50: 707–9.

    Article  PubMed  Google Scholar 

  36. Fagerberg B, Hultén LM, Hulthe J. Plasma ghrelin, body fat, insulin resistance, and smoking in clinically healthy men: the atherosclerosis and insulin resistance study. Metabolism. 2003, 52: 1460–3.

    Article  PubMed  CAS  Google Scholar 

  37. Qi X, Reed JT, Wang G, Han S, Englander EW, Greeley Jr GH. Ghrelin secretion is not reduced by increased fat mass during diet-induced obesity. Am J Physiol Regul Integr Comp Physiol 2008, 295: R429–35.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  38. Salbe AD, Tschop M, DelParigi A, Venti C, Tataranni P. Negative relationship between fasting plasma ghrelin concentrations and ad libitum food intake. J Clin Endocrinol Metab 2004, 89: 2951–6.

    Article  PubMed  CAS  Google Scholar 

  39. Katsuki A, Urakawa H, Gabazza EC, et al. Circulating levels of active ghrelin is associated with abdominal adiposity, hyperinsulinemia and insulin resistance in patients with type 2 diabetes mellitus. Eur Jour of Endocrinol 2004, 151: 573–7.

    Article  CAS  Google Scholar 

  40. Langenberg C, Bergstrom J, Laughlin GA, Barrett-Connor E. Ghrelin and the metabolic syndrome in older adults. J Clin Endocrinol Metab 2005, 90: 6448–53.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Applied Sciences, Section of Human Nutrition, University of Pavia School of Medicine, via Bassi 21, 27100, Pavia, Italy

    M. Sculati MD, PhD, H. Cena & C. Roggi

  2. Institute of Food Science and Nutrition, Cattolica University, Piacenza, Italy

    F. Rossi

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  1. M. Sculati MD, PhD
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Correspondence to M. Sculati MD, PhD.

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Sculati, M., Rossi, F., Cena, H. et al. Effect of dietary glycemic index on food intake, adiposity, and fasting plasma ghrelin levels in animals. J Endocrinol Invest 33, 250–253 (2010). https://doi.org/10.1007/BF03345788

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