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Sodium valproate does not augment Prpsc in murine neuroblastoma cells

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Abstract

Sodium valproate (VPA) has been reported to increase the accumulation of the pathologic isoform of prion protein (PrPsc) in scrapie-infected murine neuroblastoma cells. In this study, the effect of VPA on PrPsc accumulation was investigated in murine N2a neuroblastoma cells chronically infected with scrapie strain 22L (N2a-22L). No accumulation of PrPsc was detected after short-term (3 days) or long-term (21 days) treatment of N2a-22L cells with 4.8, 12, 18 or 24 μM VPA. Higher VPA concentrations (240 and 600 μM) also failed to augment PrPsc expression. In conclusion, in our experimental conditions, no deleterious effect was induced by VPA on prions replication.

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Authors and Affiliations

  1. Service de Neurovirologie, CEA, CRSSA, Université Paris XI, EPHE, IPSC, Fontenay-aux-Roses, France

    Christophe Legendre, Fabrice Casagrande, Thibault Andrieu & Domnique Dormont

  2. Laboratoire de Neurovirologie, SPI-BIO, CEA, Fontenay-aux-Roses, France

    Pascal Clayette

Authors
  1. Christophe Legendre
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  2. Fabrice Casagrande
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  3. Thibault Andrieu
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  4. Domnique Dormont
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  5. Pascal Clayette
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Corresponding author

Correspondence to Domnique Dormont.

Additional information

Dedicated to Pr. Dominique Dormont, who died in November 2003.

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Legendre, C., Casagrande, F., Andrieu, T. et al. Sodium valproate does not augment Prpsc in murine neuroblastoma cells. neurotox res 12, 205–208 (2007). https://doi.org/10.1007/BF03033916

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  • DOI: https://doi.org/10.1007/BF03033916

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