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Neurochemical and behavioural correlates in cassava-induced neurotoxicity in rats

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Abstract

Chronic cyanide intoxication from cassava has been implicated as the cause for a degenerative neuropathy known widely as tropical ataxic neuropathy. An attempt has been made in this study to identify the specific cause for neuropathy caused by cassava using Wistar strain albino rats as the experimental animal model. The results of cassava fed animals were compared with control animals, animals given cyanide, malnourished animals and malnourished animals fed cyanide, to identify the causative factors. This study revealed that though the behavioural pattern in motor coordination of the cassava fed animals was similar to the other groups studied, the neurochemical basis for the observed behavioural pattern was unique for cassava. Hence the neurotoxicity of cassava could be attributed to unmetabolized linamarin, more than its nutritional status and/or cyanide toxicity.

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References

  • Banea, M., Tylleskar, T. and Rosling, H. (1997) Konzo and Ebola in Bandundi region.Lancet 349, 621.

    Article  PubMed  CAS  Google Scholar 

  • Casadei, E., Cliff, T. and Neves, J. (1990) Surveillance of urinary thiocyanate concentration after epidemic spastic paraparesis in Mozambique.J. Trap. Med. Hyg. 93, 257–261.

    CAS  Google Scholar 

  • Clark, A. (1935) On the acetidogy of pellagra and allied nutritional diseases.West. Afr. Med. J. 8, 79.

    Google Scholar 

  • Cliff, J., Lundqvist, P., Martensson, J., Rosling, H. and Sorbo, B. (1985) Association of high cyanide and low sulphur intake in cassava-induced spastic paraparesis.Lancet 2(8466), 1211–1213.

    Article  PubMed  CAS  Google Scholar 

  • Cruickshank, E.K. (1976) Effects of malnutrition on the central nervous system and the nerves. In:Handbook of Clinical Neurology, Vol. 28. Metabolic and Deficiency Diseases of the Nervous System, Part 2. Amsterdam, Elsevier North, pp. 1–41.

  • Delange, E, Bourdoux, P., Colinet, E.et al. (1982) Nutritional factors involved in the goitrogenic action of cassava. In:Cassava Toxicity and Thyroid: Research and Public Health Issues. Proceedings of a Workshop, Ottawa IDRC Monograph 207e, pp. 17–26.

  • Dunham, N.W. and Miya, T.S. (1975) A note on a simple apparatus for detecting neurological deficit in rats and mice.J. Am. Pharmaceutical Assoc. Sci. XIVI, 208–209.

    Google Scholar 

  • Glowinski, J. and Iverson, L.L. (1966) Regional studies of catecholamines in the rat brain — I. The disposition of (3H) norepinephrine (3H) dopamine and (3H) dopa in various regions of the brain.J. Neurochem. 13, 655–669.

    Article  PubMed  CAS  Google Scholar 

  • Kamalu, B.P. (1991) The effect of nutritionally-balanced cassava (Manihot esculenta Crantz) diet on endocrine function using the dog as a mode. 2.Thyroid. Br. J. Nutr. 65, 373–379.

    CAS  Google Scholar 

  • Kanthasamy, A.G., Borowitz, J.L., Pavalkovic, G. and Isom, G.E. (1994) Dopaminergic neurotoxicity of cyanide: neurochemical, histological and behavioural characterization.Toxicol. Appl. Pharmacol. 126, 156–163.

    Article  PubMed  CAS  Google Scholar 

  • Maduagwa, E.N. and Umoh, I.B. (1986) Biliary excretion of linamarin in the wistar rat after a single dose.Biochem. Pharmacol. 35, 13003–3006.

    Google Scholar 

  • Maduagwa, E.N. (1989) Metabolism of linamarin in rats.Fd. Chem. Toxicol. 27, 451–454.

    Article  Google Scholar 

  • Mathangi, D.C., Mohan, V. and Namasivayam, A. (1999) Effect of cassava on motor coordination and neurotransmitter level in the albino rat.Fd. Chem. Toxicol. 37, 57–60.

    Article  CAS  Google Scholar 

  • Mathangi, D.C. and Namasivayam, A. (2000) Effect of chronic cyanide intoxication on memory in albino rats.Fd. Chem. Toxicol. 38, 51–55.

    Article  CAS  Google Scholar 

  • Milingi, N., Kimatta, S., Rosling, H. (1991) Konzo, a paralytic disease observed in southern Tanzania.Trop. Doct. 21, 24–25.

    Google Scholar 

  • Ministry of Health — Mozambique. Casadei, E., Janes, P., Molin, A., Rodrigues, A. and Rosling, H. (1984b) Mantakassa: an epidemic of spastic paraparesis associated with chronic cyanide intoxication in a cassava staple area in Mozambique 2. Nutritional factors and hydrocyanide content of cassava products.Bull WHO 62, 485–492.

    Google Scholar 

  • Money, G.L. (1959) Clinical aspects of tropical ataxic neuropathy due to malnutrition.West Afr. Med. J. 8, 3–6.

    PubMed  CAS  Google Scholar 

  • Nartey, F. (1968) Studies on cassavaManihot utilissima pohl I. Cyanogenesig: the biochemistry of linamarin and lotau-strolin in etiolated seedlings.Phytochemistry 7, 1307–1312.

    Article  CAS  Google Scholar 

  • Oke, O.L. (1973): The mode of cyanide detoxication. In:Chronic Cassava Toxicity. Proc. Interdisciplinary Workshop, London, IDRC 0l0e, pp. 97–104.

  • Osuntokun, B.O. (1971) Epidemiology of the tropical ataxic neuropathy in Nigeria.Trans. R. Soc. Trap. Med. Hyg. 65, 454–479.

    Article  CAS  Google Scholar 

  • Osunstokun, B.O. (1972) Chronic cyanide neurotoxicity in Nigerian plant foods.Hum. Nutr. 2, 215–260.

    Google Scholar 

  • Osuntokun, B.O. (1981) Cassava diet, chronic cyanide intoxication and neuropathy in the Nigerian Africans.Wld. Rev. Nutr. Diet. 36, 141–173.

    CAS  Google Scholar 

  • Persson, S. A., Cassel, G. and Sellstrom, A. (1985) Acute cyanide intoxication and central transmitter systems.Fund. Appl. Toxicol. 5, S150-S159.

    Article  CAS  Google Scholar 

  • Spillane, J.D. (1973) As quoted by Osuntokun (1981).

  • Tyllesker, T., Banea, M., Bikangi, N., Fresco, L., Persson, L.A. and Rosling, H. (1991) Epidemiological evidence from Zaire for a dietary etiology of konzo, an upper motor neuron disease.WHO Bull. OMS 69, 581–589.

    Google Scholar 

  • Tylleskar, T., Legue, R.N., Peterson, S., Kpizinqui, E. and Stocker, P. (1994) Konzo in central African Republic.Neurology 44, 959–961.

    PubMed  CAS  Google Scholar 

  • Umoh, I.B., Ogunkoya, F.O. and Maduagwa, E.N. (1985) Effect of thiamine status on the metabolism of linamarin in rats.Ann. Nutr. Metab. 29, 319–324.

    Article  PubMed  CAS  Google Scholar 

  • Wagner, J., Vitali, P., Palfreynman, M.G., Zraika, M. and Hout, S. (1982) Simultaneous determination of 3,4-dihydroxyphenylalanine, 5-hydroxytryptophan, dopamine, 4-hydroxy-3-ethoxyphenylalanine, norepinephrine, 3,4-dihy-droxyphenylacetic acid, homovanillic acid, serotonin and 5-hydroxy indole acetic acid in rat cerebrospinal fluid and brain by high-performance liquid chromatography with electrochemical detection.J. Neurochem. 38, 12–41.

    Article  Google Scholar 

  • Winkler, W.O. (1958) Reports on methods for glucosidal hydrogen cyanide in lima beans.J. Assoc. Agri. Chemist. 41, 282–287.

    CAS  Google Scholar 

  • Wood, T. (1965) The cyanogenic glucoside content of cassava and cassava products.J. Sci. Fd. Agric. 16, 300–305.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Physiology, Dr. ALM Postgraduate Institute of Basic Medical Sciences, University of Madras, 600113, Taramani, Chennai, India

    D. C. Mathangi & A. Namasivayam

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  1. D. C. Mathangi
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  2. A. Namasivayam
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Correspondence to A. Namasivayam.

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Mathangi, D.C., Namasivayam, A. Neurochemical and behavioural correlates in cassava-induced neurotoxicity in rats. neurotox res 2, 29–35 (2000). https://doi.org/10.1007/BF03033325

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