Abstract
Phytophthora infestans (Mont.) de Bary, the cause of late blight, has rapidly overcome major (R) gene resistance in potatoes. A population of short-day adapted tetraploid potatoes with horizontal resistance to late blight was developed at the International Potato Center in Lima, Peru. True seed from this population was obtained from the NRSP-6 Project at Sturgeon Bay, WI for the purpose of evaluating its potential to contribute to the breeding effort for late blight resistance in the United States. In 1996, 552 single hills were planted on Chapman Farm, Presque Isle, ME; only 448 tuberized. In 1997, these clones were planted on Chapman Farm for increase; 129 were saved, 53 failed to tuberize, and 266 were discarded because they were sprouted at harvest. In 1998 and 1999, 69 clones were tested for their reaction to late blight in replicated plots on Aroostook Farm, Presque Isle, ME. Percent infected foliage was estimated three times toward the end of the season and used to compute area under the disease progress curve. Broad-sense heritability for horizontal resistance to late blight was estimated as 0.78 with a 95% confidence interval of 0.64 to 0.86. Using detached leaflet assays, these clones were inoculated with US-8 strains ofP. infestans, and the diameter of the lesion was measured 7, 8, 9, 10 and 11 days after inoculation. The correlation between field resistance and the detached leaflet assay was very low (0.18<r<0.24). Some clones from this population are highly resistant to the US-8 strain of P.infestans and represent another source of breeding material for developing late blight resistant varieties in the future. However, very late maturity, short dormancy, poor tuberization and low fertility levels may limit the usefulness of this germplasm in northern USA latitudes.
Resumen
Phytophthora infestans (Mont.) de Bary, causante del tizón tardío, ha vencido rápidamente la resistencia de genes mayores (R) en papa. El Centro Internacional de la Papa en Lima, Perú, ha desarrollado una población de papa tetraploide adaptada a días cortos, con resistencia horizontal al tizón. La semilla verdadera (botánica) de esta población fue obtenida del Proyecto NRSP-6 en Sturgeon Bay, WI, con el propósito de evaluar su potencial para contribuir con el mejoramiento para resistencia a tizón tardío en los Estados Unidos. En 1996, se sembraron para multiplicación 552 plantas en la granja Chapman, Isla Presque, ME, de estas, solo tuberizaron 448. En 1997 estos clones se sembraron en la granja Chapman para incremento, 129 se guardaron, 53 no tuberizaron y se eliminaron 266 porque brotaron a la cosecha. En 1998 y 1999, se probaron 69 clones para su reacción al tizón tardío en parcelas repetidas en la granja Aroostook, Isla Presque, ME. El porcentaje de follaje infectado fue estimado por trés veces hacia el final de la campaña y usado para calcular el área bajo la curva de progreso de la enfermedad. La herencia en sentido amplio para resistencia al tizón tardio fue estimada como 0.78 con un 95% de intervalo de confianza de 0.64 a 0.86. Utilizando pruebas de foliolos desprendidos, estos clones fueron inoculados con variantes US-8 deP. infestans y se midió el diámetro de la lesión 7, 8, 9,10 y 11 días después de la inoculación. La correlación fue muy baja (0.18<r<0.24). Algunos clones de esta población son muy resistentes a la variante US-8 deP. infestans y représentan otra fuente de material de mejoramiento para desarrollar variedades resistentes al tizón tardío en el futuro. Sin embargo, la madurez muy tardía, periodo de latencia corto, tuberización pobre y bajos nivelés de fertilidad podrían limitar la utilidad de este germoplasma en latitudes del norte de los Estados Unidos de América.
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Abbreviations
- AUDPC:
-
area under the disease progress curve
- sAUDPC:
-
square root of AUDPC
- H:
-
broad-sense heritability
- Disclaimer:
-
Reported use of brand name products does not imply an endorsement by USDA
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Haynes, K.G., Goth, R.W., Lambert, D.H. et al. Evaluation of a short-day adapted tetraploid potato population with horizontal resistance toPhytophthora infestans under long-day conditions in northern Maine. Amer J of Potato Res 84, 459–466 (2007). https://doi.org/10.1007/BF02987882
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DOI: https://doi.org/10.1007/BF02987882