Abstract
We have previously reported on the identification of the endogenous transmethylation inhibitor oligosaccharide-linked acyl carrier protein (O-ACP). In this study, the role of the transmethylation reaction on cell cycle progression was evaluated using various transmethylase inhibitors, including O-ACP. O-ACP significantly inhibited the growth of various cancer cell lines, including NIH3T3,ras-transformed NIH3T3, MDA-MB-231, HT-1376, and AGS. In addition, exposure ofras-transformed NIH3T3 to O-ACP caused cell cycle arrest at the Go/G1, phase, which led to a decrease in cells at the S phase, as determined by flow cytometry. In contrast, transmethylase inhibitors did not affect the expression of p21WAF1/Cip1, a well known inhibitor of cyclin dependent kinase, indicating that the cell cycle arrest by transmethylase inhibitors might be mediated by a p21WAF1/Cip1-independent mechanism. Therefore, O-ACP, a novel transmethylase inhibitor, could be a useful tool for elucidating the novel role of methylation in cell proliferation and cell cycle progression.
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Seo, D.W., Kim, Y.K., Cho, EJ. et al. Oligosaccharide-linked acyl carrier protein, a novel transmethylase inhibitor, from porcine liver inhibits cell growth. Arch Pharm Res 25, 463–468 (2002). https://doi.org/10.1007/BF02976603
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DOI: https://doi.org/10.1007/BF02976603