Abstract
To investigate the efficacy of vinblastine as a possible therapeutic agent in prolactinomas, we have examined the effects of vinblastine on GH3 cell function. The effects of vinblastine were compared to another anti-microtubule drug, nocodazole. At 24 h, prolactin (PRL) secretion was 737±63 ng/ml in control cells. In cells treated with 0.1, 1 and 10μm nocodazole for 24 h, PRL secretion was reduced to 200±30 ng/ml. After a 24 h incubation with the drugs, cells were washed with drug-free medium and challenged with 100nm TRH for 10 min. TRH-stimulated PRL secretion was 35±7 ng/ml in control cells, 14±0.5 ng/ml in vinblastine-treated cells and 8.8±0.1 ng/ml in nocodazole-treated cells. [3H]TRH binding to GH3 cell membrane was inhibited by about 15% by vinblastine and nocodazole. In vinblastine and nocodazole treated cells, polymerized tubulin levels decreased by 46 and 55%, respectively. These observations that vinblastine suppresses PRL secretion by GH3 cells suggest that this drug might be useful as a therapeutic agent for prolactinomas.
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Ravindra, R., Forman, L.J. & Patel, S.A. Vinblastine and nocodazole inhibit basal and thyrotropin-releasing hormone-stimulated prolactin secretion in GH3 cells. Endocr 3, 591–596 (1995). https://doi.org/10.1007/BF02953024
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DOI: https://doi.org/10.1007/BF02953024