Abstract
Foliar spray and micro-injection of plant growth-promoting rhizobacterial species,viz. Pseudomonas fluorescens andP. aeruginosa on chickpea induced synthesis of phenylalanine ammonia-lyase (PAL) when tested againstSclerotinia sclerotiorum. Induction of PAL was also associated with increased synthesis of phenolic compounds such as tannic, gallic, caffeic, chlorogenic and cinnamic acids. Treatment withP. fluorescens was found to be more effective in inducing phenolic compounds as compared toP. aeruginosa. However, persistence of PAL activity was observed more withP. aeruginosa. Although both the inoculation methods were effective, foliar application was found to be superior to micro-injection in terms of rapid PAL activity leading to the synthesis of phenolic compounds.
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Abbreviations
- HPLC:
-
high performance liquid chromatography
- IAA:
-
3-indolylacetic acid
- ISR:
-
induced systemic resistance
- KB:
-
King’s B (agar)
- Pf4 :
-
Pseudomonas fluorescens
- Pag :
-
Pseudomonas aeruginosa
- PAL:
-
phenylalanine ammonia-lyase (EC 4.3.1.5)
- PDA:
-
potato-dextrose agar
- PGPR:
-
plant growth-promoting rhizobacteria
- Sst :
-
Sclerotinia sclerotiorum
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Basha, S.A., Sarma, B.K., Singh, D.P. et al. Differential methods of inoculation of plant growth-promoting rhizobacteria induce synthesis of phenylalanine ammonia-lyase and phenolic compounds differentially in chickpca. Folia Microbiol 51, 463–468 (2006). https://doi.org/10.1007/BF02931592
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DOI: https://doi.org/10.1007/BF02931592