Abstract
Immunoelectrophoresis and its modifications were applied to analysis of a lipopolysaccharide-like component (LPS-LC) extracted fromBorrelia garinii strains K24 and K48 isolated fromIxodes ricinus andBorrelia burgdorferi sensu stricto strain B31. A modification of the hot phenol-water method was used for isolation of LPS. Immunoelectrophoresis (IE) and crossed immunoelectrophoresis (CIE) of LPS-LC with polyclonal rabbit antisera revealed a pattern and properties partially similar to LPS from other Gramnegative bacteria.B. garinii LPS-LC formed in CIE a diffuse band extending from the start to the anode. Similarly, the shape and position of the band in IE did not show major differences from LPS of other Gramnegative bacteria. The LPS-LC extracted from the three genomic groups ofB. burgdorferi sensu lato were found to have similar immunochemical properties irrespective of their genotype origin.
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Reference
Axelsen N.H., Kroll J., Weeke B.:A Manual of Quantitative Immunoelectrophoresis. Methods and Applications, Vol. 169. Indre Smaalenenes Trykkeri, Mysen (Norway) 1973.
Baranton G., Postic D., Saint Girons I., Boerlin P., Piffaretti J.C., Assous M., Grimont P.A.D.: Delineation ofBorrelia burgdorferi sensu stricto,Borrelia garinii sp.nov., and group VS461 associated with Lyme borreliosis.Internat.J.Syst. Bacteriol.42, 378–383 (1992).
Beck G., Habicht G.S., Benach J.L., Coleman J.L.: Chemical and biologic characterization of a lipopolysaccharide extracted from the Lyme disease spirochete (Borrelia burgdorferi).J.Infect.Dis.152, 108–117 (1985).
Bukovský M., Čižnár I.: Immunochemical properties ofVibrio cholerae LPS.Folia Microbiol.38, 519–523 (1993).
Burgdorfer W.A., Barbour A.G., Hayes S.F., Benach J.L., Grunwaldt E., Davis J.P.: Lyme disease, a tick-borne spirochetosis?Science216, 1317–1319 (1982).
Eiffert H., Lotter H., Jarecki-Khan K., Thomssen R.: Identification of an immunoreactive nonproteinaleous component inBorrelia burgdorferi.Med.Microbiol.Immunol.180, 229–237 (1991).
Giambartolomei G.H., Dennis V.A., Lasater B.L., Murthy P.K., Philipp M.T.: Autocrine amd exocrine regulation of interleukin-10 production in THP-1 cells stimulated withBorrelia burgdorferi lipoproteins.Infect.Immun.70, 1881–1888 (2002).
Hanincová K., Taragelová V., Kočí J., Schafer S.M., Hails R., Ullmann A.J., Piesman J., Labuda M., Kurtenbach K.: Association ofBorrelia garinii andB. valaisiana with songbirds in Slovakia.Appl.Environ.Microbiol.69, 2825–2830 (2003).
Heroldova M., Němec M., Hubálek Z., Halouzka J.: Enzyme activities ofBorrelia burgdorferi sensu lato.Folia Microbiol.46, 179–182 (2001).
Hindersson P., Thomas D., Stamm L., Penn C., Norris S., Joens L.A.: Interaction of spirochetes with the host.Res.Microbiol.143, 629–639 (1992).
Laemmli V.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature227, 680–685 (1970).
Longworth D.L.: The clinical challenge of Lyme disease.Cleve Clin.J.Med.57, 453–463 (1990).
Postic D., Assous M.V., Grimont P.D., Baranton G.: Diversity ofBorrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism ofrrf(5S)-rrl(23S) intergenic spacer amplicons.Internat.J.Syst.Bacteriol.44, 743–752 (1994).
Schwarzová K., Čižnár I.: Combined infection ofIxodes ricinus with threeBorrelia burgdorferi sensu lato genetypes.Folia Microbiol.49, 297–300 (2004).
Schwarzová K., Čižnár I.: Spirochetal non-Borrelia microorganism isolated fromIxodes ricinus.Folia Microbiol.41, 175–180 (1996).
Sellati T.J., Burns M.J., Ficazzola M.A., Furie M.B.:Borrelia burgdorferi upregulates expression of adhesion molecules on endothelial cells and promotes transendothelial migration of neutrophilsin vitro.Infect.Immun.63, 4439–4447 (1995).
Steere A.C., Grodzicki R.L., Kornblatt A.N., Craft J.E., Barbour A.G., Burgdorfer W., Schmid G.P., Johnson E., Malawista S.E.: The spirochetal etiology of Lyme disease.N.Engl.J.Med.308, 733–740 (1983).
Taborský L., Adam P., Sobek O., Dostál M., Dvořáková J., Dubská L.: Levels of apolipoprotein A-II in cerebrospinal fluid in patients with neuroborreliosis are associated with lipophagocytosis.Folia Microbiol.48, 849–855 (2003).
Takayama K., Rothenberg R.J., Barbour A.G.: Absence of lipopolysaccharide in the Lyme disease spirochete.Borrelia burgdorferi. Infect.Immun.55, 2311–2313 (1987).
Tatro J.B., Romero L.I., Beaslez D., Steere A.C., Reichlin S.:Borrelia burgdorferi andEscherichia coli lipopolysaccharides induce nitric oxide and interleukin-6 production in cultured rat brain cells.J.Infect.Dis.169, 1014–1022 (1993).
Van Damm A.P., Kuiper H., Vos K., Widjojokusumo A., De Jongh B.M., Spanjaard L., Ramselaar A.C.P., Kramer M.D., Dankert J.: Different genospecies ofBorrelia burgdorferi are associated with distinct clinical manifestation of Lyme borreliosis.Clin.Infect.Dis.17, 708–717 (1993).
Westphal O., Jann K.: Extraction with phenol-water and further applications of the procedure, pp. 83–91 inMethods in Carbohydrate Chemistry, Vol. 5 (R.L. Whistler, M.L. Wolfrom, Eds). Academic Press, New York 1965.
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Schwarzová, K., Čižnár, I. Immunochemical analysis of lipopolysaccharide-like component extracted fromBorrelia burgdorferi sensu lato. Folia Microbiol 49, 625–629 (2004). https://doi.org/10.1007/BF02931545
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DOI: https://doi.org/10.1007/BF02931545