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The effect of fluidic conditions on the continuous-flow bioluminescent detection of ATP in a microfluidic device

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Abstract

The effect of fluidic conditions on the bioluminescent detection of ATP in a microfluidic device was surveyed using homemade detector system. The bioluminescent reaction of ATP was directly affected by the retention time and flow rates of the solutions in this diffusion-based mixing and reaction system due to the laminar flow in the microchannel. ATP and enzyme solutions were separately injected into the microfluidic device at different flow rates through 3 inlet ports. The ATP solution was injected through the middle port, while the enzyme solution was injected in the two remaining ports. When the ratio of ATP to enzyme solution was fixed, the optimum flow rates of enzyme, ATP, and enzyme solution was 3.5, 8.0, and 3.5 μL/min, respectively. The optimal total flow rate was 15 μL/min. The detection limit for the concentration of ATP at optimal conditions was less than 10−7 M.

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Authors and Affiliations

  1. Department of Biological Engineering, Inha University, 402-751, Incheon, Korea

    Thuan-Hieu Tran, Young-Bum Kim, Yoon-Mo Koo & Eun-Ki Kim

  2. ERC for Advanced Bioseparation Technology, Inha University, 402-751, Incheon, Korea

    Woo-Jin Chang & Yoon-Mo Koo

  3. M&B GreenUs Co., Ltd., 152-021, Seoul, Korea

    Joo-Young Yoon

  4. Department of Polymer Science and Engineering, Sungkyunkwan University, 440-746, Suwon, Korea

    Jinhwan Kim

Authors
  1. Thuan-Hieu Tran
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  2. Woo-Jin Chang
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  3. Young-Bum Kim
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  4. Yoon-Mo Koo
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  5. Eun-Ki Kim
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  6. Joo-Young Yoon
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  7. Jinhwan Kim
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Correspondence to Eun-Ki Kim or Jinhwan Kim.

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Tran, TH., Chang, WJ., Kim, YB. et al. The effect of fluidic conditions on the continuous-flow bioluminescent detection of ATP in a microfluidic device. Biotechnol. Bioprocess Eng. 12, 470–474 (2007). https://doi.org/10.1007/BF02931342

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  • DOI: https://doi.org/10.1007/BF02931342

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