Abstract
Enzyme immunoassay (EIA) using recombinant antigens for the detection ofTreponema pallidum-specific antibodies in sera of syphilis patients was developed. Four low-molar-massTreponema antigens (Tp15, Tp17, TmpA, Tp47) were investigated; 17- and 47-kDa proteins were demonstrated as immunodominant as they permitted to obtain the most sensitive EIA. Using a mixture of these proteins a 3rd-generation-EIA kit Dia-Syph was constructed, its sensitivity being 99.4% during tests of 165 sera of syphilitic patients. No false result was obtained on the commercial panel PSS01 (BBI, USA). The specificity of the elaborated test system (99.7%) was determined on 295 sera.
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Rostopira, N., Tkáčiková, L., Rayevska, G. et al. Elaboration of enzyme immunoassay based on recombinant antigens and intended for diagnostics of syphilis. Folia Microbiol 48, 549–553 (2003). https://doi.org/10.1007/BF02931339
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DOI: https://doi.org/10.1007/BF02931339