Abstract
The presence of insertion elements (IS)IS861 andIS1548 in the collection of 211Streptococcus agalactiae strains isolated from pregnant women and dairy cows was assayed.IS861 was found in 67 human strains (59%) and 36 bovine strains (37%),IS1548 in 13 human strains (12%) and 16 bovine strains (16%). Two combinations,IS861+ IS1548− andIS861− IS1548−, were widely distributed in both human and bovine strains. The copy number and the restriction fragment length polymorphism (RFLP) of the two IS were determined in human group B streptococcus (GBS) strains. A minimum of 8 copies ofIS1548 were detected in GBS strains while the copy number ofIS861 varied from 1 to 9. The number of different hybridizing patterns withIS861 andIS1548 probes was 9 and 6, respectively. These hybridization patterns were divided into several clusters. All strains with IS were also clustered according to pulsed fieldgel electrophoresis (PFGE) patterns. A correlation was found between the results of PFGE- and IS-based clustering.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chatellier S., Huet H., Kenzi S., Rosenau A., Geslin P., Quentin R.: Genetic diversity of rRNA operons of unrelatedStreptococcus agalactiae strains isolated from cerebrospinal fluid of neonates suffering from meningitis.J.Clin.Microbiol. 34, 2741–2747 (1996).
Dmitriev A., Tkáčiková L’., Suvorov A., Kantiková M., Mikula I., Totolian A.: Comparative genetic study of group B streptococcal strains of human and bovine origin.Folia Microbiol. 44, 449–453 (1999).
Dmitriev A., Totolian A., Tkáčiková L’., Mikula I.: Features of the genome structure of group B streptococci of bovine origin.Folia Vet. 45, 57–63 (2001).
Dmitriev A., Shakleina E., Tkáčiková L’., Mikulá I., Totolian A.: Genetic heterogeneity of the pathogenic potentials of human and bovine group B streptococci.Folia Microbiol. 47, 291–295 (2002).
Elliot J.A., Farmer K.D., Facklam R.R.: Sudden increase in isolation of group B streptococci, serotype V, is not due to emergence of a new pulsed-field gel electrophoresis type.J.Clin.Microbiol. 36, 2115–2116 (1998).
Ferrieri P., Cho D.S., Livdahl C., Rubens C.E., Flores A.E.: DNA restriction profiles of nontypable group B streptococci, predominantly in strains causing endocarditis.J.Infect.Dis. 177, 967–976 (1998).
Hauge M., Jespersgaard C., Poulsen K., Kilian M.: Population structure ofStreptococcus agalactiae reveals an association between specific evolutionary lineages and putative virulence factors but not disease.Infect.Immun. 64, 919–925 (1996).
Hoe N., Nakashima K., Grigsby D., Pan X., Dou S.J., Naidich S., Garcia M., Kahn E., Bergmire-Sweat D., Musser J.M.: Rapid molecular genetic subtyping of serotype M1 group A streptococcus strains.Emerg.Infect.Dis. 5, 254–263 (1999).
Keefe G.P.:Streptococcus agalactiae mastitis: a review.Can.Vet.J. 38, 429–437 (1997).
Mahillon J., Chandler M.: Insertion sequences.Microbiol.Mol.Biol.Rev. 62, 725–774 (1998).
Maniatis T., Fritsch E.F., Sambrook J.:Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor (USA) 1982.
Martinez G., Harel J., Higgins R., Lacouture S., Daignault D., Gottschalk M.: Characterization ofStreptococcus agalactiae isolates of bovine and human origin by randomly amplified polymorphic DNA analysis.J.Clin.Microbiol. 38, 71–78 (2000).
Quentin R., Huet H., Wang F.-S., Geslin P., Goudeau A., Selander R.K.: Characterization ofStreptococcus agalactiae strains by multilocus enzyme genotype and serotype: identification of multiple virulent clone families that cause invasive neonatal diseases.J.Clin.Microbiol. 33, 2576–2581 (1995).
Regan J.A., Klebanoff M.A., Nugent R.P., Eschenbach D.A., Blackwelder W.C., Lou Y., Gibbs R.S., Rettig P.J., Martin D.H., Edelman R.: Colonization with group B streptococci in pregnancy adverse outcome. VIP study group.Am.J.Obstet.Gynecol. 174, 1354–1360 (1996).
Rolland K., Marois C., Siquier V., Cattier B., Quentin R.: Genetic features ofStreptococcus agalactiae strains causing severe neonatal infections, as revealed by pulsed-field gel electrophoresis andhylB gene analysis.J.Clin.Microbiol. 37, 1892–1898 (1999).
Rubens C.E., Heggen L.M., Kuypers J.M.:IS861, a group B streptococcal insertion sequence related toIS150 andIS3 ofEscherichia coli.J.Bacteriol. 171, 5531–5535 (1989).
Suvorov A., Dmitriev A., Ustinovich I., Schalen C., Totolian A.: Molecular analysis of clinical group B streptococcal strains by use of α and β gene probes.FEMS Immunol.Med.Microbiol. 17, 149–154 (1997).
Tenover F.C., Arbeit R.D., Goering R.V., Mickelsen P.A., Murray B.E., Persins D.H., Swaminsthan B.: Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis; criteria for bacterial strain typing.J.Clin. Microbiol. 33, 2233–2239 (1995).
Tinsley C.R., Nassif X.: Analysis of the genetic differences betweenNeisseria meningitidis andNeisseria gonorrhoeae: two closely related bacteria expressing two different pathogenicities.Proc.Nat.Acad.Sci.USA 93, 11109–11114 (1996).
Author information
Authors and Affiliations
Corresponding author
Additional information
This study was supported by funding of theMinistry of Science and Technology (P.R.China), by theRussian Foundation for Basic Research grants 00-04-49360a and 02-04-06927, and by theSlovak Grant Agency VEGA grant 1-8021-01.
Rights and permissions
About this article
Cite this article
Dmitriev, A., Yang, M., Shakleina, E. et al. The presence of insertion elementsIS861 andIS1548 in group B streptococci. Folia Microbiol 48, 105–110 (2003). https://doi.org/10.1007/BF02931285
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02931285