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Characterization ofCandida albicans colony-morphology mutants and their hybrids

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Abstract

The collection wild-type strain ofCandida albicans was used to obtain auxotrophic and colony-morphology mutants by ‘nitrosoguanidine’ treatment. Intraspecific protoplast fusion induced by Ca2+-poly(ethyleneglycol) was carried out in various pairings between the auxotrophic strain producing smooth colonies and containing blastospores and the colony-morphology mutants containing a mixture of blastospores and pseudohyphae or only hyphae. Hybrids exhibiting full or partial complementation were obtained when mutants producing smooth colonies and colony-morphology variants of different origins were fused. The mutation responsible for the colony-morphology character (if various types of colony morphomutants were crossed) proved to be recessive or semidominant. Representative hybrids exhibited elevated DNA contents as measured by flow cytometry. To illustrate various cell types, and especially the intermediate one (never observed in natural isolates), a preparation method was further developed for scanning electron microscopic studies.

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References

  • Barnes W.G., Flesher A., Berger A.E., Arnold J.D.: Scanning electron microscopic studies ofCandida albicans.J.Bacteriol. 106, 276–280 (1971).

    PubMed  CAS  Google Scholar 

  • Braun B.R., Johnson A.D.: Control of filament formation inCandida albicans by the transcriptional repressor TUP1.Science 277, 105–109 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Calderone R.A., Fonzi W.A.: Virulence factors ofCandida albicans.Trends Microbiol. 9, 327–335 (2001).

    Article  PubMed  CAS  Google Scholar 

  • Dorko E., Jenča A., Pilipčinec E., Danko J., Švický E., Tkáčiková L’.:Candida-associated denture stomatitis.Folia Microbiol. 46, 443–446 (2001a).

    Article  CAS  Google Scholar 

  • Dorko E., Virágová S., Jautová J., Pilipčinec E., Danko J., Švický E., Tkáčiková L’.: Electrophoretic karyotyping ofCandida albicans strains isolated from premature infants and hospital personnel in a neonatal intensive care unit.Folia Microbiol. 46, 453–458 (2001b).

    Article  CAS  Google Scholar 

  • Dorko E., Pilipčinec E., Tkačiková L’.:Candida species isolated from cerebrospinal fluid.Folia Microbiol. 47, 179–181 (2002a).

    Article  CAS  Google Scholar 

  • Dorko E., Jautová J., Tkáčiková L’., Wantrubová A.: The frequency ofCandida species in onychomycosis.Folia Microbiol. 47, 727–731 (2002b).

    Article  CAS  Google Scholar 

  • Ferenczy L., Maráz A.: Transfer of mitochondria by protoplast fusion inSaccharomyces cerevisiae.Nature 268, 524–525 (1977).

    Article  PubMed  CAS  Google Scholar 

  • Fournier P., Provost A., Bourguignon C., Heslot H.: Recombination after protoplast fusion in the yeastCandida tropicalis.Arch. Microbiol. 115, 143–149 (1977).

    Article  PubMed  CAS  Google Scholar 

  • Fujita T., Tokunaga J., Inoue H.:Atlas of Scanning Electron Microscopy in Medicine. Igaku Shoin Ltd., Tokyo 1971.

    Google Scholar 

  • Gil C., Pomes R., Nombela C.: A complementation analysis by parasexual recombination ofCandida albicans morphological mutants.J.Gen.Microbiol. 134, 1587–1595 (1988).

    PubMed  CAS  Google Scholar 

  • Haidaris C.G., Malone J., Sherril L.A., Bliss J.M., Gaspary A.A., Insel R.A., Sullivan M.A.: Recombinant human antibody single chain variable fragments reactive withCandida albicans surface antigens.J.Immunol.Meth. 257, 185–202 (2001).

    Article  CAS  Google Scholar 

  • Hilton C., Markie D., Corner B., Rikkerink E., Poulter R.: Heat shock induces chromosome loss in the yeastCandida albicans.Mol.Gen.Genet. 200, 162–168 (1985).

    Article  PubMed  CAS  Google Scholar 

  • Hubbard M.J., Markie D., Poulter R.T.M.: Isolation and morphological characterization of a mycelial mutant ofCandida albicans.J.Bacteriol. 165, 61–65 (1986).

    PubMed  CAS  Google Scholar 

  • Joshi K.R., Wheeler E.E., Garin J.B.: Scanning electron microscopy of colonies of six species ofCandida.J.Bacteriol. 115, 341–348 (1973).

    PubMed  CAS  Google Scholar 

  • Köhler J.R., Fink G.R.:Candida albicans strains heterozygous and homozygous for mutations in mitogen-activated protein kinase signaling components have defects in hyphal development.Proc.Nat.Acad.Sci.USA 93, 13223–13228 (1996).

    Article  PubMed  Google Scholar 

  • Nombela C., Pomes R., Gil C.: Protoplast fusion hybrids fromCandida albicans morphological mutants.Crit.Rev.Microbiol. 15, 79–85 (1987).

    PubMed  CAS  Google Scholar 

  • Novák A., Vágvölgyi C., Fekete-Forgács K., Pesti M.: Isoenzyme and RAPD-PCR analysis and adhesion to acrylic of the hybrids ofCandida albicans morphological mutants.Folia Microbiol. 46, 248 (2001).

    Google Scholar 

  • Odds F.C.: Candidaand Candidosis, 2nd ed. Baillere Tindall, London 1988.

    Google Scholar 

  • Odds F.C.:Candida species and virulence.ASM News 60, 313–318 (1994).

    Google Scholar 

  • Payton M.A., Detiani M.: The isolation of osmotic remedial conditional lethal mutants ofCandida albicans.Curr.Genet. 17, 293–296 (1990).

    Article  PubMed  CAS  Google Scholar 

  • Pesti M., Ferenczy L.: Protoplast fusion hybrids ofCandida albicans sterol mutants differing in nystatin resistance.J.Gen.Microbiol. 128, 123–128 (1982).

    PubMed  CAS  Google Scholar 

  • Pesti M., Sipiczki M., Pintér Y.: Scanning electron microscopy characterization of colonies ofCandida albicans morphological mutants.J.Med.Microbiol. 48, 167–172 (1999).

    PubMed  CAS  Google Scholar 

  • Pesti M., Hidasi J., Novák A.: Complementation analysis of colony morphological mutants ofCandida albicans.Folia Microbiol. 45, 84 (2000).

    Google Scholar 

  • Pesti M., Vágvölgyi Cs., Papp T., Nagy A., Novák A.: Variation of isoenzyme and RAPD patterns inCandida albicans morphological mutants with altered colony ultrastructure.Acta.Biol.Hung. 52, 289–298 (2001).

    Article  PubMed  CAS  Google Scholar 

  • Pla J., Gil C., Monteoliva L., Navarro-Garcia F., Sanchez M., Nombela C.: UnderstandingCandida albicans at the molecular level.Yeast 12, 1677–1702 (1996).

    Article  PubMed  CAS  Google Scholar 

  • Pomes R., Concepcion G., Cabetas M.D., Nombela C.: Variability of colonial morphology in benomyl-induced morphological mutants fromCandida albicans.FEMS Microbiol. Lett. 48, 255–259 (1987).

    Article  CAS  Google Scholar 

  • Poulain D., Hopwood V., Vernes A.: Antigenic variability ofCandida albicans.Crit.Rev.Microbiol. 12, 223–270 (1985).

    Article  PubMed  CAS  Google Scholar 

  • Poulter R., Jeffery K., Hubbard M.J., Shepherd M.G., Sullivan P.A.: Parasexual genetic analysis ofCandida albicans by spheroplast fusion.J.Bacteriol. 146, 833–840 (1981).

    PubMed  CAS  Google Scholar 

  • Radford D.R., Challacombe S.J., Walter J.D.: A scanning electron microscopy investigation of the structure of colonies of different morphologies produced by phenotypic switching ofCandida albicans.J.Med.Microbiol. 40, 416–423 (1994).

    PubMed  CAS  Google Scholar 

  • Rutschenko-Bulgac E.P.: Variations ofCandida albicans electrophoretic karyotypes.J.Bacteriol. 20, 6586–6596 (1991).

    Google Scholar 

  • Rutschenko-Bulgac E.P., Curran T.M., Sherman F.: Variations in the number of ribosomal DNA units in morphological mutants and normal strains ofCandida albicans and in normal strains ofSaccharomyces cerevisiae.J.Bacteriol. 715, 7189–7199 (1993).

    Google Scholar 

  • San-Blas G., Travassos L.R., Fries B.C., Goldman D.L., Casadevall A., Carmona A.K., Barros T.F., Puccia R., Hostetter M.K., Shanks S.G., Copping V.M.S., Knox Y., Gow N.A.R.: Fungal morphogenesis and virulence.Med.Mycol. 38, 79–86 (2000).

    PubMed  Google Scholar 

  • Sarachek A., Rhoads D.D., Schwarzhoff R.H.: Hybridization ofCandida albicans through fusion of protoplasts.Arch.Microbiol. 129, 1–8 (1981).

    Article  PubMed  CAS  Google Scholar 

  • Sherer S., Magee P.T.: Genetics ofCandida albicans.Microbiol.Rev. 54, 226–241 (1990).

    Google Scholar 

  • Slutsky B., Staebell M., Anderson J., Risen L., Pfaller M., Soll D.R.: “White-opaque transition”: a second high-frequency switching system inCandida albicans.J.Bacteriol. 169, 189–197 (1987).

    PubMed  CAS  Google Scholar 

  • Soll D.R.: High frequency switching inCandida albicans.Clin.Microbiol.Rev. 5, 183–203 (1992).

    PubMed  CAS  Google Scholar 

  • Stoldt V.R., Sonneborn A., Lenker C.E., Ernst J.F.: Efg1p, an essential regulator of morphogenesis of the human pathogenCandida albicans, is a member of a conserved class of bHLH proteins regulating morphogenetic processes in fungi.EMBO J. 16, 1982–1991 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Stöver A.G., Witek-Janusek L., Mathews H.L.: A method for flow cytometric analysis ofCandida albicans DNA.J.Microbiol.Meth. 33, 191–196 (1998).

    Article  Google Scholar 

  • Szaniszlo P.J., Jacobs C.W., Geis P.A.: Dimorphism: morphological and biochemical aspects, pp. 323–436 in D.H. Howard (Eds):Fungi Pathogenic for Humans and Animals, Part A. Marcel Dekker, New York 1983.

    Google Scholar 

  • Takasuka T., Baily G.G., Birch M., Anderson M.J., Lew D., Denning D.W.: Variation in morphotype, karyotype and DNA type of fluconazole-resistantCandida albicans from an AIDS patient.J.Infect. 36, 57–62 (1998).

    Article  PubMed  CAS  Google Scholar 

  • Tomšíková A.: Causative agents of nosocomial mycoses.Folia Microbiol. 47, 105–112 (2002).

    Article  Google Scholar 

  • Vallin C., Ferenczy L.: Diploid formation ofCandida tropicalis via protoplast fusion.Acta Microbiol.Acad.Sci.Hung. 25, 209–212 (1978).

    PubMed  CAS  Google Scholar 

  • Whittaker D.K., Drucker D.B.: Scanning electron microscopy of intact colonies of microorganisms.J.Bacteriol. 104, 902–909 (1970).

    PubMed  CAS  Google Scholar 

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Novák, A., Vágvölgyi, C. & Pesti, M. Characterization ofCandida albicans colony-morphology mutants and their hybrids. Folia Microbiol 48, 203–209 (2003). https://doi.org/10.1007/BF02930957

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