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Multicentric focal acinar cell hyperplasia and hepatocyte-like cell metaplasia are induced by nitrosomethylurea in rat pancreas

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Summary

The present report is a study of the effect of the carcinogen nitrosomethylurea (NMU) on pancreas of rats receiving during lifetime a lipid-poor diet, that is essential fatty acid deficient or control diets. Rats fed a commercial stock chow were mated. At day 10 of pregnancy, dams were divided into three groups, that were respectively supplied with the commercial chow, the essential fatty acid deficient or the sufficient diet. Each litter was separated at random in two groups that received at day one of life one intraperitoneal injection of NMU (50 mg/kg b.w.) or saline. After weaning, they were maintained for life with the diet that was supplied to their mothers. The pancreas of NMU-treated rats presented diffuse proliferative changes, focal acinar cell hyperplasias (FACH), and focal hepatocyte-like metaplasia (FHLCM). FACH were expansive presumably preneoplastic growths, showing abnormal differentiation. The number of NMU-treated rats bearing FACH and FHLCM did not significantly differ in the three nutritional conditions.

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Abbreviations

EFAD:

Essential fatty acid deficient diet

EFAS:

Essential fatty acid sufficient diet

FACH:

Focal acinar cell hyperplasia

FHLCM:

Focal hepatocytee-like cell metaplasia

NMU:

Nitrosomethylurea

ST:

Stock diet

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Authors and Affiliations

  1. Institute de Biologia Celular, Córdoba University School of Medicine and CONICET, Casilla de Correo 220, 5000, Cordoba, Argentina

    Benito Mortis, Mirta A. Valentich, Raúl Urrutia & Marcelo Rivolta

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  1. Benito Mortis
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  2. Mirta A. Valentich
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  3. Raúl Urrutia
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  4. Marcelo Rivolta
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Mortis, B., Valentich, M.A., Urrutia, R. et al. Multicentric focal acinar cell hyperplasia and hepatocyte-like cell metaplasia are induced by nitrosomethylurea in rat pancreas. Int J Pancreatol 8, 119–131 (1991). https://doi.org/10.1007/BF02924426

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  • DOI: https://doi.org/10.1007/BF02924426

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