Abstract
Protoplasts ofBacillus subtilis 168trpC2 str and cells ofEscherichia coli SK1590 after treatment with calcium chloride were transformed to tetracycline resistance with the recombinant plasmid pUN82 entrapped in the reverse phase evaporation hposomes. Frequency of transfer was 4×10-4 % inB. subtilis and 8×10-6 % inE. coli.
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References
Chang A. C. Y., Cohen S. N.: High frequency transformation ofB. subtilis protoplasts by plasmid DNA.Mol. Gen. Genet. 168, 111 (1979).
Dagert M., Ehrlich S. D.: Prolonged incubation in calcium chloride improves the competence ofE. coli cells.Gene 6, 23 (1979).
Eckhardt T.: A rapid method for the identification of plasmid deoxyribonucleic acid in bacteria.Plasmid 1, 584 (1978).
Fraley R., Fornari C., Kaplan S.: Entrapment of a bacterial plasmid in phospholipid vesicles -potential for gene transfer.Proc. Nat. Acad. Sci. USA 76, 3348 (1979).
Fraley R., Subramani S., Berg P., Papahadjopoulos D.: Introduction of liposome-encapsu-lated SV40 DNA into cells.J. Biol. Chem. 255, 10431 (1980).
Fraley R., Papahadjopoulos D.: Liposomes: The development of a new carrier system for introducing nucleic acids into plant and animal cells.Curr. Topics Microbiol. Immunol. 96, 171 (1982).
Holubová I., Hubáček J., Ludvík J.: Transformation of calcium treatedEscherichia coli cells with liposome-encapsulated plasmid DNA, Abstract A16 at3rd Symp. Socialist Countries on Biotechnology, Bratislava (Czechoslovakia) 1983.
Humphreys G. O., Willshaw G. A., Anderson E. S.: A simple method for the preparation of large quantities of pure plasmid DNA.Biochim. Biophys. Acta 383, 457 (1975)
Makins J. F., Holt G.: Liposome-mediated transformation of streptomycetes by chromosomal DNA.Nature 293, 671 (1981).
Nicolau C., Sene C.: Liposome-mediated DNA transfer in eukaryotic cells. Dependence of the transfer efficiency upon the type of liposomes used and the host cell cycle stage.Biochim. Biophys. Acta 72l, 185 (1982).
Nicolau C., Soriano P., Le Pape A., Londos-Gagliabdi D., Legband A., Grosse E.: Liposomes as carriers for gene transferin vivo.Biol. Cell 47, 121 (1983).
Rizzo W. B., Schulman J. D., Mukherjee A. B.: Liposome-mediated transfer of Simian virus 40 DNA and minichromosome into mammalian cells.J. Gen. Virol. 64, 911 (1983).
Schahfer-Ridder M., Wang Y., Hofschneider P. H.: Liposome as gene carriers: efficient transformation of mouse L cells by thymidine kinase gene.Science 215, 166 (1982).
Schaffer P., Cami B., Hotchkiss R. D.: Fusion of bacterial protoplasts.Proc. Nat. Acad. Sci. USA 73, 2151 (1976).
Szoka F., Papahadjopoulos D.: Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation.Proc. Nat. Acad. Sci. USA 74, 4194 (1978).
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Holubová, I., Jandová, Z., Tichý, P. et al. Transfer of liposome-encapsulated plasmid DNA toBacillus subtilis protoplasts and calcium-treatedEscherichia coli cells. Folia Microbiol 30, 97–100 (1985). https://doi.org/10.1007/BF02922200
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DOI: https://doi.org/10.1007/BF02922200