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Bone marrow analysis of the myelodysplastic syndromes: histological and immunohistochemical features related to the evolution of overt leukemia

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Virchows Archiv B

Summary

Bone marrow trephines from 31 patients with an initial diagnosis of myelodysplastic syndromes (MDS) were examined and analyzed histologically and immunohistochemically. In those cases terminating in overt leukemia (6/31, 19%), the number of bone marrow mast cells was significantly reduced, compared with those which did not evolve to overt leukemia. The bone marrow lymphoid cells that may participate in immunosurveillance against the proliferation of blast cells were also significantly reduced in cases terminating in overt leukemia. However, S-100 protein-positive cells, which include histiocytes and suppressor T-cells, were increased in cases terminating in overt leukemia. The results indicated that examination of the bone marrow to determine the proportions of mast cells and lymphoid cells which may be involved in host defense systems may be useful in predicting the evolution to overt leukemia in MDS. In the present series, patients with a hypocellular marrow (5/31, 16%) did not progress to overt leukemia and had a significantly lower bone marrow reticulin content, a significantly lower megakaryocyte count, a relatively higher mast cell count and a significantly higher lymphoid cell count than those with a normocellular or hypercellular marrow. These findings may reflect the initial features of MDS or, possibly, that hypocellular MDS is an independent entity with a low potential for biastic proliferation.

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References

  • Anderson RL, Bagby GC (1982) The prognostic value of chromosomal studies in patients with the primary preleukemia syndrome (hemopoietic dysplasia). Leuk Res 6:175–181

    Article  PubMed  CAS  Google Scholar 

  • Ascensas JL, Kay NE, Wright JJ, Arthur D, Finkel B, Rydell R, Kaplan ME (1986) Lymphoblastic transformation of myelodysplastic syndrome. Am J Hematol 22:431–434

    Article  Google Scholar 

  • Barton JC, Conrad ME, Parmley RT (1980) Acute lymphoblastic leukemia in idiopathic sideroblastic anemia: evidence for a common lymphoid and myeloid progenitor cell. Am J Hematol 9:109–115

    Article  PubMed  CAS  Google Scholar 

  • Bauermeister DE (1971) Quantitation of bone marrow reticulin-a normal range. Am J Clin Pathol 56:24–31

    PubMed  CAS  Google Scholar 

  • Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DAG, Gralnick HR, Sultan C (1982) Proposals for the classification of the myelodysplastic syndromes. Br J Haematol 51:189–199

    PubMed  CAS  Google Scholar 

  • Brito-Babapulle F, Catovsky D, Galton DAG (1987) Clinical and laboratory features ofde novo acute myeloid leukaemia with trilineage myelodysplasia. Br J Haematol 66:445–450

    Article  PubMed  CAS  Google Scholar 

  • Bynoe AG, Scott CS, Ford P, Roberts BE (1983) Decreased T helper cells in the myelodysplastic syndromes. Br J Haematol 54:97–102

    Article  PubMed  CAS  Google Scholar 

  • Chiao JW, Freitag WF, Steinmetz JC, Andreff M (1981) Changes of cellular markers during differentiation of HL-60 promyelocytes to macrophage as induced by T-lymphocyte conditioned medium. Leuk Res 5:477–490

    Article  PubMed  CAS  Google Scholar 

  • Clark R, Peters S, Hoy T, Smith S, Whittaker K, Jacobs A (1986) Prognostic significance of hypoploid haematopoietic precursors in myelodysplastic syndromes. N Engl J Med 314:1472–1475

    PubMed  CAS  Google Scholar 

  • Cline MJ, Golde DW (1979) Cellular interactions in haematopoiesis. Nature 277:177–181

    Article  PubMed  CAS  Google Scholar 

  • Delacretaz F, Schmidt P-M, Piguet D, Bachmann F, Costa J (1987) The FAB classification (proposals) applied to bone marrow biopsy. Am J Clin Pathol 87:180–186

    PubMed  CAS  Google Scholar 

  • Dörmer P, Hershko C, Wilmanns W (1987) Mechanisms and prognostic value of cell kinetics in the myelodysplastic syndromes. Br J Haematol 67:147–152

    Article  PubMed  Google Scholar 

  • Fenaux P, Jouet JP, Zandecki M, Lai JL, Simon M, Pollet JP, Bauters F (1987) Chronic and subacute myelomonocytic leukaemia in the adult: a report of 60 cases with special reference to prognostic factors. Br J Haematol 65:101–106

    Article  PubMed  CAS  Google Scholar 

  • Fohlmeister I, Fischer R, Mödder B, Rister M, Schaefer H-E (1985 a) Aplastic anaemia and the hypocellular myelodysplastic syndrome: histomorphological, diagnostic, and prognostic features. J Clin Pathol 38:1218–1224

    Article  PubMed  CAS  Google Scholar 

  • Fohlmeister I, Fischer R, Schaefer H-E (1985b) Preleukemic myelodysplastic syndromes (MDS): Pathogenetical considerations based on retrospective clinicomorphological sequential studies. Anticancer Res 5:179–188

    PubMed  CAS  Google Scholar 

  • Fohlmeister I, Reber T, Fisher R (1985 c) Bone marrow mast cell reaction in preleukemic myelodysplasia and in aplastic anemia. Virchows Arch [Pathol Anat] 405:503–509

    Article  CAS  Google Scholar 

  • Foucar K, Langdon RM, Armitage JO, Olson DB, Carroll TJ (1984) Myelodysplastic syndromes. A clinical and pathologic analysis of 109 cases. Cancer 56:553–561

    Article  Google Scholar 

  • Frisch B, Bartl R (1986) Bone marrow histology in myelodysplastic syndromes. Scand J Haematol [Suppl] 45:21–37

    CAS  Google Scholar 

  • Goordyal P, Isaacson PG (1985) Immunocytochemical characterization of monocyte colonies of human bone marrow: a clue to the origin of Langerhans cells and interdigitating reticulum cells. J Pathol 146:189–195

    Article  PubMed  CAS  Google Scholar 

  • Hall PA, Lindeman R, Butler MG, Amess JAL, D’ardenne AJ (1987) Demonstration of lymphoid antigens in decalcified bone marrow trephines. J Clin Pathol 40:870–873

    Article  PubMed  CAS  Google Scholar 

  • Hamaguchi Y, Kanakura Y, Fujita J (1987) Interleukin 4 as an essential factor for in vitro clonal growth of murine connective tissue type mast cells. J Exp Med 165:268–273

    Article  PubMed  CAS  Google Scholar 

  • Hokland P, Kerndrup G, Griffin JD, Ellegaard J (1986) Analysis of leukocyte differentiation antigens in blood and bone marrow from preleukemia (refractory anemia) patients using monoclonal antibodies. Blood 67:898–902

    PubMed  CAS  Google Scholar 

  • Hsu S-M, Reine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques. J Histochem Cytochem 29:577–580

    PubMed  CAS  Google Scholar 

  • Hussein KK, Salem Z, Bottmley SS, Livingstone RB (1982) Acute leukemia in idiopathic sideroblastic anemia: response to combination chemotherapy. Blood 59:652–656

    PubMed  CAS  Google Scholar 

  • Jacobs A (1986) Myelodysplastic syndromes: pathogenesis, functional abnormalities, and clinical implications. J Clin Pathol 38:1201–1217

    Article  Google Scholar 

  • Jacobs A (1987) Human preleukemia: Do we have a model? Br J Cancer 55:1–5

    PubMed  CAS  Google Scholar 

  • Jacobs RH, Cornbleet MA, Vardiman JW, Larson RA, Le Beau MM, Rowley JD (1986) Prognostic implications of morphology and karyotype in primary myelodysplastic syndromes. Blood 67:1765–1772

    PubMed  CAS  Google Scholar 

  • Kitagawa M, Matsubara O, Kasuga T (1986) Dynamics of lymphocytic subpopulations in Friend leukemia virus-induced leukemia. Cancer Res 46:3034–3039

    PubMed  CAS  Google Scholar 

  • Knox SJ, Greenberg BR, Anderson RW, Rosenblatt LS (1983) Studies of T-lymphocytes in preleukemic disorders and acute nonlymphocytic leukemia: In vitro radiosensitivity, mitogenic responsiveness, colony formation, and enumeration of lymphocytic subpopulations. Blood 61:449–455

    PubMed  CAS  Google Scholar 

  • Nagler A, Hazani A, Etzioni A, Tatarsky I (1988) Distribution of T lymphocyte subsets preceding refractory anemia with excess blasts in transformation in a child. Acta Haematol 79:103–106

    PubMed  CAS  Google Scholar 

  • Peters SW, Clark RE, Hoy TG, Jacobs A (1986) DNA content and cell cycle analysis of bone marrow cells in myelodysplastic syndromes (MDS). Br J Haematol 62:239–245

    Article  PubMed  CAS  Google Scholar 

  • Poppema S, Hollema H, Visser L, Vos H (1987) Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin-embedded tissue sections. Am J Pathol 127:418–429

    PubMed  CAS  Google Scholar 

  • Prokocimer M, Polliack A (1981) Increased bone marrow mast cells in preleukemic syndromes, acute leukemia, and lymphoproliferative disorders. Am J Clin Pathol 75:34–38

    PubMed  CAS  Google Scholar 

  • Raskind WH, Tirumali N, Jacobson R, Singer J, Failkow PJ (1984) Evidence for a multistep pathogenesis of a myelodysplastic syndrome. Blood 63:1318–1323

    PubMed  CAS  Google Scholar 

  • Ribera J-M, Cervantes F, Rozman C (1987) A multivariate analysis of prognostic factors in chronic myelomonocytic leukaemia according to the FAB criteria. Br J Haematol 65:307–311

    Article  PubMed  CAS  Google Scholar 

  • Roche WR (1986) The nature and significance of tumour-associated mast cells. J Pathol 148:175–182

    Article  PubMed  CAS  Google Scholar 

  • Sansoni P, Rowden G, Manara GC, Ferrari C, Panfilis GD (1987) S-100-positive T cells are largely restricted to a CD8positive, 9.3-negative subset. Virchows Arch [Cell Pathol] 53:301–307

    Article  CAS  Google Scholar 

  • Todd WM, Pierre RV (1986) Preleukaemia: A long-term prospective study of 326 patients. Scand J Haematol [Suppl] 45:114–120

    CAS  Google Scholar 

  • Tricot G, Wolf-Peters CD, Hendrickx B, Verwilghen RL (1984a) Bone marrow histology in myelodysplastic syndromes. I. Histological findings in myelodysplastic syndromes and comparison with bone marrow smears. Br J Haematol 57:423–430

    Article  PubMed  CAS  Google Scholar 

  • Tricot G, Wolf-Peters CD, Vlietinck R, Verwilghen RL (1984b) Bone marrow histology in myelodysplastic syndromes. II. Prognostic value of abnormal localization of immature precursors in MDS. Br J Haematol 58:217–225

    Article  PubMed  CAS  Google Scholar 

  • Tricot G, Boogaerts MA, Wolf-Peters CD, Van den Berghe H, Verwilghen RL (1985) The myelodysplastic syndromes: different evolution patterns based on sequential morphological and cytogenetic investigations. Br J Haematol 59:659–670

    Article  PubMed  CAS  Google Scholar 

  • Vallespi T, Torrabadella M, Julia A, Irriguible D, Jaen A, Acebedo G, Triginer J (1985) Myelodysplastic syndromes: a study of 101 cases according to the FAB classification. Br J Haematol 61:83–92

    Article  PubMed  CAS  Google Scholar 

  • Vanstapel MJ, Peeters B, Cordeil J, Heyne W, Wolf-Peters DC, Desmet V, Mason DY (1985) Production of monoclonal antibodies directed against antigenic determinants common to the end chain of bovine brain S-100 protein. Lab Invest 52:232–238

    PubMed  CAS  Google Scholar 

  • Yoo D, Lessin LS, Jensen WN (1978) Bone marrow mast cells in lymphoproliferative disorders. Ann Intern Med 88:753–757

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, Faculty of Medicine, Tokyo Medical and Dental University 5-45, Yushima 1-chome, 113, Tokyo, Japan

    Masanobu Kitagawa, Ryuichi Kamiyama & Tsutomu Kasuga

  2. Department of Pathology, Japan Red Cross Medical Center, Japan

    Tamiko Takemura

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  1. Masanobu Kitagawa
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  2. Ryuichi Kamiyama
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  3. Tamiko Takemura
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  4. Tsutomu Kasuga
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Kitagawa, M., Kamiyama, R., Takemura, T. et al. Bone marrow analysis of the myelodysplastic syndromes: histological and immunohistochemical features related to the evolution of overt leukemia. Virchows Archiv B Cell Pathol 57, 47–53 (1989). https://doi.org/10.1007/BF02899064

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  • DOI: https://doi.org/10.1007/BF02899064

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