Skip to main content
SpringerLink
Log in

Kinetic studies of the murine foetal thymus using vincristine sulphate

  • Published:
Virchows Archiv B

Summary

The turnover time of the foetal thymus has been evaluated in CD1 mice using the metaphase arrest drug vincristine sulphate and also by direct cell counting and found to be 18 h (range 12–26) and 11.9 h (range 10.9–13.1) respectively. Vincristine sulphate can be used for cell kinetic studies on foetal thymus provided an appropriate dose (5 mgm per kgm body weight given intravenously) and time scale (less than 1 hour after injection) are used for these measurements. These conditions are different from those used for adult tissues. Using125I-iododeoxyuridine uptake measurements, it was found that vincristine sulphate suppressed DNA synthesis in the foetal thymus but not in the maternal thymus at this dose. Only the G2 cohort of cells in the thymus entered mitosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abercrombie M (1946) Estimation of nuclear population from microtome sections. Anat Rec 94: 239

    Article  Google Scholar 

  • Aherne WA, Camplejohn RS (1972) On correcting the error due to metaphase degeneration in stathmokinetic studies. Exp Cell Res 74: 496

    Article  PubMed  CAS  Google Scholar 

  • Aherne WA, Camplejohn RS, Wright NA (1977)An introduction to cell population kinetics. Arnold, London

    Google Scholar 

  • Al-Dewachi HS, Wright NA, Appleton DR, Watson AJ (1975) Cell population kinetics in the mouse jejunal crypt. Virchows Arch [Cell Pathology] 18: 225

    CAS  Google Scholar 

  • Ball WD (1963) A quantitative assessment of mouse thymus differentiation. Exp Cell Res 31: 82

    Article  PubMed  CAS  Google Scholar 

  • Clarke RM (1971) A comparison of metaphase arresting agents and tritiated thymidine autoradiography in measurement of the rate of entry of cells into mitosis in the crypts of Lieberkuhn of the rat. Cell. Tissue Kinet 4: 263

    PubMed  CAS  Google Scholar 

  • Cleaver JE (1967)Thymidine metabolism and cell kinetics. North Holland, Amsterdam

    Google Scholar 

  • Duffill MB, Appleton DR, Dyson P, Shuster S, Wright NA (1977) The measurement of the cell cycle time in squamous epithelium using the metaphase arrest technique with vincristine. Br J Dermatol 96: 493

    Article  PubMed  CAS  Google Scholar 

  • Eigsti OJ, Dustin P (1955)Colchicine in Agriculture, Medicine, Biology and Chemistry. Iowa State College Press, Ames, Iowa

    Google Scholar 

  • Fabrikant JI, Foster BR (1972) Cell population kinetics in lymphocytes in mouse and rat thymus. John Hopkins Med J 130: 208

    CAS  Google Scholar 

  • Hell E, Cox DG (1963) Effects of colchicine and colcemid on synthesis of deoxyribose nucleic acid in skin of the guinea pig ear in vitro. Nature 197: 287

    Article  PubMed  CAS  Google Scholar 

  • Mandel, TE, Kennedy MM (1978) The differentiation of murine thymocytes in vivo and in vitro. Immunology 35: 317

    PubMed  CAS  Google Scholar 

  • Metcalf D, Wiadrowski M (1966) Autoradiographic analysis of lymphocyte proliferation in the thymus and in thymic lymphoma tissue. Cancer Res 26: 483

    PubMed  CAS  Google Scholar 

  • Michalke WD, Hess MW, Riedwyl H, Stoner RD, Cottier H (1969) Thymic lymphopoiesis and cell loss in newborn mice. Blood 33: 541

    PubMed  CAS  Google Scholar 

  • Micklem HS (1972) Cell proliferation in haematopoietic spleen colonics of mice: difference between colonies derived from injected adult bone marrow and foetal liver cells. Cell Tissue Kinet 5: 159

    PubMed  CAS  Google Scholar 

  • Nome O (1975) Demecolcin and vinblastine sulphate as stathmokinetic agents for different tissues of hairless mouse. Pathol Europ 10: 221

    CAS  Google Scholar 

  • Philp JR, Buchanan TJ (1971) Quantitative measurement on finite tissue sections. J Anat 108: 89

    PubMed  CAS  Google Scholar 

  • Pritchard H, Micklem HS (1972) Immune responses in congenitally thymus-less mice. I. Absence of response to oxazolone. Clin Exp Immunol 10: 151

    PubMed  CAS  Google Scholar 

  • Riches AC, Gore D, Docherty J, Littlewood V (1976) The use of 5-[125I]-iodo-21-deoxyuridine for monitoring DNA synthesis in organ culture. J Anat 121: 323

    PubMed  CAS  Google Scholar 

  • Smith SR, Thomas DB, Riches AC (1974) Cell production in tumor isografts measured using vincristine and colcemid. Cell Tissue Kinet 7: 529

    PubMed  CAS  Google Scholar 

  • Tannock IF (1965) A comparison of the relative efficiencies of various metaphase arrest agents. Exp Cell Res 47: 345

    Article  Google Scholar 

  • Taylor EW (1965) The mechanism of colchicine inhibition of mitosis J. Cell Biol 25: 145

    Article  PubMed  CAS  Google Scholar 

  • Taylor L, Carlile AE, Thomas DB, Riches AC (1977) Kinetic studies on the thymus. J Anat 123: 251

    Google Scholar 

  • Thomas DB (1974) The Cell in Medical Science, Volume 2. Academic Press, London

    Google Scholar 

  • Thorud E, Clausen OPF, Bjerknes R, Aarnaes E (1980) The stathmokinetic method in vivo - time response with special reference to circadian variations in epidermal cell proliferation in the hairless mouse. Cell Tissue Kinet 13: 625

    PubMed  CAS  Google Scholar 

  • Williams JPG (1968) Inhibition of embryonic deoxytibonucleic acid synthesis by colcemid. Eur J Pharmacol 3: 337

    Article  PubMed  CAS  Google Scholar 

  • Williams JPG (1970) Selective inhibition of embryonic deoxyribonucleic acid synthesis by vinblastine. Cell Tissue Kinet 3: 155

    PubMed  CAS  Google Scholar 

  • Williams JPG, Carpentieri U (1967) The different response of embryonic and adult rats to demecolcin. Life Sciences 6: 2613

    Article  PubMed  CAS  Google Scholar 

  • Wright NA, Al-Dewachi HS, Appleton DR, Watson AJ (1975) Cell population kinetics in the rat jejunal crypt. Cell Tissue Kinet 8: 361

    PubMed  CAS  Google Scholar 

  • Wright NA, Appleton DR (1980) The metaphase arrest technique - a critical review. Cell Tissue Kinet 13: 643

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy and Experimental Pathology, Bute Medical Buildings, University of St Andrews, KY16 9TS, St Andrews, Scotland

    Andrew C. Riches, Howard M. H. Carr, Linda McQueen & David Brynmor Thomas

Authors
  1. Andrew C. Riches
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Howard M. H. Carr
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Linda McQueen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. David Brynmor Thomas
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Riches, A.C., Carr, H.M.H., McQueen, L. et al. Kinetic studies of the murine foetal thymus using vincristine sulphate. Virchows Archiv B Cell Pathol 37, 297–307 (1981). https://doi.org/10.1007/BF02892578

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02892578

Key words

Search

Navigation