Summary
The expression of surface antigens on both normal adult’s bone marrow and multipotential progenitor cells (CFU-Mix) was detected by use of ABC technique. The culture system of CFU-Mix was an ideal model for studying the differentiation direction of hematopoietic cells. The monoclonal antibodies (McAb) CD3, CD4, CD8specific for T-lymphocytic lineage, CD22 for B-lymphocytic lineage and CD11, CD13, CD14 for granulocytomonocytic lineage were all demonstrated in certain proportion in cultured CFU-Mix, which might be associated with the control of hematopoiesis. A decrease in the positive rate of OKT, and CD34 in cultured cells might be related to the differentiation of hematopoietic cells. SZ-2 and SZ-21 could contribute to the identification of the presence of megakaryocytes in CFU-Mix.
Similar content being viewed by others
References
1988;9:527.
1987;10:459.
Ferrer D, et al. Surface phenotypes of human hemapoietic progenitor cells defined by monoclonal antibodies. Blood 1985;66:496.
Foon KA, et al. Immunologic classification of leukemia and lymphoma. Blood 1986;68:1.
1990;13:30.
Lebman D, et al. A monoclonal antibody that detects expression of transferrin receptor in human erythroid precursor cells. Blood 1982;59:671.
1987;67:76.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yan, C., Dong-jiao, Y., Chong-yu, L. et al. Detection of surface antigen expression of pre- and postcultured normal adult’s bone marrow cells by using ABC technique and its significance. Journal of Tongji Medical University 12, 179–182 (1992). https://doi.org/10.1007/BF02887821
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02887821