Skip to main content
SpringerLink
Log in

An experimental study on the regulation of expression of Th2 cytokines from T lymphocytes by protein kinase C in asthma

  • Published:
Current Medical Science Aims and scope Submit manuscript

Summary

To explore the regulatory role of protein kinase C (PKC) in the expression of Th2 cytokines, interleukin-4 (IL-4) and interleukin-5 (IL-5) by T lymphocytes in asthma. T lymphocytes were isolated and purified from blood and bronchial alveolus lavage fluid (BALF) of each guinea pig of normal control group and asthmatic group and from peripheral blood of the asthmatic patients and normal controls, and were stimulated with PKC accelerant phorbol 12-myristate 13-acetate (PMA) and inhibitor Ro31-8220. The expression of IL-4 and IL-5 mRNA and protein was detected by using in situ hybridization staining and ELISA respectively. The expression of IL-4 and IL-5 mRNA and protein of asthmatic T lymphocytes stimulated with PMA was significantly higher than that of asthmatic T lymphocytes stimulated without PMA respectively (P<0. 01) and that of normal T lymphocytes stimulated with PMA respectively (P<0. 01). The expression of IL-4 and IL-5 mRNA and protein of asthmatic T lymphocytes stimulated with PMA and Ro31-8220 was significantly lower than that of asthmatic T lymphocytes stimulated only with PMA respectively (P<0. 01). It was concluded that PKC might participate in regulating the expression of IL-4 and IL-5 in asthmatic T lymphocytes, and the activation of PKC in T lymphocytes might play an important role in the pathogenesis of asthma.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Hogan S P; Koskinen A, Matthaei K Iet al. Interleukin-5-producing CD4 + T cells play a pivotal role in aeroallergen-induced eosinophilia, bronchial hyperteactivity, and lung damage in mice. Am J Respir Crit Care Med, 1998, 157:210

    Article  CAS  PubMed  Google Scholar 

  2. 1999, 22:461

  3. 1998, 21:208

  4. 1997, 20:261

  5. Monks C R F, Kupfer H, Tamie Iet al. Selective modulation of protein kinase C during T cell activation. Nature, 1997, 385:83

    Article  ADS  CAS  PubMed  Google Scholar 

  6. Hehner S P, Li-Weber M, Giaisi Met al. Vav synergizes with protein kinase C theta to mediate IL-4 gene expression in response to CD28 costimulation in T cells. J Immunol, 2000, 164:3829

    Article  CAS  PubMed  Google Scholar 

  7. Roberts A I, Brolin R E, Ebert E C. Integrin alphalbetal (VLA-1) mediates adhesion of activated intraepithelial lymphocytes to collagen. Immunology, 1999, 97:679

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Bahattacharyya S P, Drucker I, Roshef Tet al. Activated T lymphocytes induce degranulation and cytokine production by human mast cells following cell-to-cell contact. J Leukocyte Biol, 1998, 63:337

    Article  Google Scholar 

  9. Bansal S K, Jha A, Jaiswal A Set al. Increased levels of protein kinase C in lymphocytes in asthma: possible mechanism of regulation. Eur Respir J, 1997, 10:308

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Respiratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan

    Xiong Weining M. D., Ph. D, Xu Yongjian, Zhang Zhenxiang, Wang Xiaoyang, Mo Biwen & Fu Juan

Authors
  1. Xiong Weining M. D., Ph. D
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xu Yongjian
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhang Zhenxiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wang Xiaoyang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mo Biwen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Fu Juan
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This project was supported by a grant from National Natural Sciences foundation (No. 30070332) and Education Ministry college cadreman teachers imbursement plan (2000 year).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Weining, X., Yongjian, X., Zhenxiang, Z. et al. An experimental study on the regulation of expression of Th2 cytokines from T lymphocytes by protein kinase C in asthma. Current Medical Science 21, 292–296 (2001). https://doi.org/10.1007/BF02886560

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02886560

Key words

Search

Navigation