Skip to main content
SpringerLink
Log in

Inhibition of the growth of human hepatoma cell line bothin vitro andin vivo by transducing CKI genep21 WAF-1 with GE7 targeting gene delivery system

  • Published:
Science in China Series C: Life Sciences Aims and scope Submit manuscript

Abstract

The EGF receptor-mediated targeting gene delivery system GE7 was used to transduce exogenous genepCEP-p21 WAF-1 into human hepatocellular carcinoma cell bothin vitro andin vivo. Afterin vitro transduction of the exogenous gene, the growth of the cell lines SMMC-7721 and BEL-7402 was significantly inhibited compared with the control. On day 8 the inhibition rates of the above cell lines reached 56.0% and 66.7%, respectively. Thein vivo experiment showed that the growth of human hepatoma transplanted in nude mice injected with GE7 gene delivery system subcutaneously once a week for 3 weeks was remarkably inhibited compared with that of untransfected control. The average tumor weight of the experiment group was (0.083 ±0.043) g, while that of the control group was (0.281 ±0.173) g. The difference is significant (P<0.05). It was indicated that GE7 gene delivery system could efficiently transduce exogenous genepCEP-p21 WAF-1 into hepatoma cell with high EGF receptor expression, and inhibit the cell growth with high efficacy bothin vivo andin vitro.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Tian, P. K., Ren, S. J., Ren, C. C. et al., A novel receptor-targeted gene delivery system for cancer gene therapy, Science in China, Ser. C, 1999, 42(2): 216.

    Article  CAS  Google Scholar 

  2. Midoux, P., Mendes, C., Legrand, A. et al., Specific gene transfer mediated by lactosylated poly-L-lysine into hepatoma cells, Nucleic Acids Res., 1993, 21: 871.

    Article  PubMed  CAS  Google Scholar 

  3. Yamaguchi, K., Carr, B. I., Nalesnik, M. A., Concomitant and isolated expression of TGF-alpha and EGF-R in human hepatoma cells supports the hypothesis of autocrine, paracrine, and endocrine growth of human hepatoma, J. Surg. Oncol., 1995, 58(4): 240.

    Article  PubMed  CAS  Google Scholar 

  4. Su, Q., Liu, Y. F., Expression of c-erbB-2 protein and EGF receptor in hepatitis B, cirrhosis and hepatocellular carcinoma, Chin. J. Pathol. (in Chinese), 1995, 24(2): 93.

    CAS  Google Scholar 

  5. Xu, Y. H., Jiang, W. L., Peng, S. F., EGFR expression and EGF stimulation of proliferation in human liver carcinoma cells, Acta Biol. Exp. Sini. (in Chinese), 1989, 22(4): 445.

    CAS  Google Scholar 

  6. Serrano, M., Hannon, G. J., Beach, D., Anew regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4, Nature, 1993, 366: 704.

    Article  PubMed  CAS  Google Scholar 

  7. Xiong, Y., Johnson, C. V., Moen, P. T. Jr. et al., P21 is a universal inhibitor of cyclin kinases, Nature, 1993, 366: 701.

    Article  PubMed  CAS  Google Scholar 

  8. Ren, C. H., Tian, P. K., Qu, S. M. et al., Expression of cyclin-dependent kinase inhibitor genes induces apoptosis in human hepatoma cell line, Chinese Science Bulletin, 1997, 42(23): 2428.

    Article  Google Scholar 

  9. Levine, A. J., p53, the cellular gatekeeper for growth and division, Cell, 1997, 88: 323.

    Article  PubMed  CAS  Google Scholar 

  10. Hollstein, M., Rice, K., Greenblatt, M. S. et al., Database of p53 gene somatic mutations in human tumors and cell lines, Nucleic Acids Res., 1994, 22: 3551.

    PubMed  CAS  Google Scholar 

  11. Wagner, E., Cotton, M., Foisner, R. et al., Transferrin-polycation-DNA complexes: the effect of polycations on the structure of the complex and DNA delivery to cells, Proc. Natl. Acad. Sci. USA, 1991, 88: 4255.

    Article  PubMed  CAS  Google Scholar 

  12. Bui, L. A., Butterfield, L. H., Kim, J. Y. et al.,In vivo therapy of hepatocellular carcinoma with a tumor-specific adenoviral vector expressing interleukin-2, Hum. Gene Ther., 1997, 8: 2173.

    Article  PubMed  CAS  Google Scholar 

  13. Xu, G. W., Sun, Z. T., Forrester, K. et al., Tissue-specific growth suppression and chemo-sensitivity promotion in human hepatocellular carcinoma cells by retroviral-mediated transfer of the wild-type p53 gene, Hepatology, 1996, 24: 1264.

    Article  PubMed  CAS  Google Scholar 

  14. Kanai, F., Lan, K. H., Shiratori, Y. et al.,In vivo gene therapy for alpha-fetoprotein-producing hepatocellular carcinoma by adenovirus-mediated transfer of cytosine deaminase gene, Cancer Res., 1997, 57: 461.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Shanghai Cancer Institute, 200032, Shanghai, China

    Junsong Han, Peikun Tian, Xiang Liu, Ming Yao & Jianren Gu

Authors
  1. Junsong Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Peikun Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ming Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jianren Gu
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Han, J., Tian, P., Liu, X. et al. Inhibition of the growth of human hepatoma cell line bothin vitro andin vivo by transducing CKI genep21 WAF-1 with GE7 targeting gene delivery system. Sci. China Ser. C.-Life Sci. 43, 663–668 (2000). https://doi.org/10.1007/BF02882288

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02882288

Keywords

Search

Navigation