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Sequencing and bacterial expression of a novel murine alpha interferon gene

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Abstract

A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed inEscherichia coli under the control of the PL promoter which resulted in antiviral activity on mouse L-cells. The sequence of mIFN-αB has been accepted by GENEBANK.

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Authors and Affiliations

  1. Shanghai Institute of Biochemistry, Chinese Academy of Sciences, 200031, Shanghai, China

    Wang Yan, Wang Zhengyu, Zhou Mingnan, Cai Ju’e, Sun Lanying & Liu Xinyuan

  2. Department of Molecular Genetics and Microbiology, UMDNJ-Robert Wood Johnson Medical School, New Jersey, USA

    B. L. Daugherty & S. Pestka

Authors
  1. Wang Yan
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  2. Wang Zhengyu
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  3. Zhou Mingnan
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  4. Cai Ju’e
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  5. Sun Lanying
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  6. Liu Xinyuan
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  7. B. L. Daugherty
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  8. S. Pestka
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Wang, Y., Wang, Z., Zhou, M. et al. Sequencing and bacterial expression of a novel murine alpha interferon gene. Sci. China Ser. C.-Life Sci. 40, 277–283 (1997). https://doi.org/10.1007/BF02879088

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  • DOI: https://doi.org/10.1007/BF02879088

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