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Effect of sodium arsenite on the biosynthesis of mitomycins byStreptomyces caespitosus and mode of action of mitomycin C onBacillus subtilis NRRL B-543

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Abstract

Addition of different concentrations of sodium arsenite to the fermentation medium vised for the production of mitomycin antibiotics byStreptomyces caespitosus hindered the biosynthesis of mitomycins and led to the accumulation of 2-oxoglutarate, pyruvate and acetone. Mitomycin C isolated and purified using thin-layer chromatography in low concentration of about 0.1 μg/ml did not affect the RNA, DNA and protein biosynthesis of the growingBacillus subtilis, while at 10 μg/ml mitomycin C markedly affected RUA, DNA and protein biosynthesis.

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Authors and Affiliations

  1. Microbiological and Enzyme Chemistry Research Unit, National Research Centre, and National Information and Documentation Centre, Dokki, Cairo, A.R. Egypt

    A. A. Abou-Zeid & A. A. Yousef

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  1. A. A. Abou-Zeid
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  2. A. A. Yousef
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Abou-Zeid, A.A., Yousef, A.A. Effect of sodium arsenite on the biosynthesis of mitomycins byStreptomyces caespitosus and mode of action of mitomycin C onBacillus subtilis NRRL B-543. Folia Microbiol 21, 36–42 (1976). https://doi.org/10.1007/BF02879004

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  • DOI: https://doi.org/10.1007/BF02879004

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