Abstract
The proposed method includes primary and secondary treatments. Of the various disinfectants tested, 1-propanol and 1-butanol as a primary treatment and phenol, resorcinol, HgCl2 or NaCl as a secondary treatment completely eliminated the contamination hazard. 1-Butanol and phenol were not useful disinfectants since they inhibited the growth ofC. fusiformis along with the other contaminants. Primary and secondary treatment of sclerotia with 1-propanol and resorcinol, respectively, produced the maximum of stable cultures. Out of 120 cultures tested, 5 cultures demonstrated an appreciable yield of alkaloids under submerged cultural conditions.
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Desai, J.D., Desai, A.J. & Vaidya, H.C. A new method for isolation of saprophytic cultures ofClaviceps fusiformis from sclerotia. Folia Microbiol 27, 182–185 (1982). https://doi.org/10.1007/BF02877398
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DOI: https://doi.org/10.1007/BF02877398