Abstract
Detection of lgE and lgG antibodies in Aspergillosis is of diagnostic significance. The serological methods, such as agglutination, gel diffusion and counter immuno electrophoresis that are commonly used in the laboratories for diagnosis of Aspergillus induced infections, are less sensitive and high cross reactivity is often encountered. We carried out work on characterization and identification of diagnostically relevant antigens ofA. fumigatus. Well characterized antigens were used to develop an ELISA with 92% sensitivity and 89% specificity for detection of specific lgE and lgG in the sera of patients of allergic bronchopulmonary aspergillosis (ABPA), aspergilloma and invasive aspergillosis. Subsequently, a sample kit having “ready to use type” of dry reagents (powder/tableted buffers and lyophilized antigen, conjugate and reference sera) was formulated. The kit was validated with sera from patients of ABPA, related allergic disorders, tuberculosis, post-Kochs cases and thalassemic children receiving repeated blood transfusions. The performance of the kit was found to be satisfactory with coded sera.
Similar content being viewed by others
References
Koll, B.S. and Brown, A.E. (1993) The changing epidemiology of infections at cancer hospitals. Clin. Inf. Dis. 17, S322-S328.
Grammer, I.D., Greenberger, P.A. and Patterson, R. (1986) Allergic bronchopulmonary aspergillosis in asthmatic patients presenting with allergic rhinitis. Int. Arch. Aller. Appl. Immunol. 79, 246–248.
Baur, X. and Dexheimer, E. (1984) Hypersensitivity pneumonitis concomitant with acute airway obstruction after exposure to hay dust. Respir. 46, 354–361.
De Coster, A., Dierckx, P. and Grivignee, A. (1988) Aspergilloma. In: Aspergillus and Aspergillosis, Eds. Bossche, H. V., Mackenzie, D.W.R. and Cauwenbergh, G. Plenum Press, NY, p 107–114.
Denning, D.W. and Stevens, D.A. (1990) Antifungal and surgical treatment of invasive aspergillosisreview of 2112 published cases. Rev. Inf. Dis. 12, 1147–1201.
Meyer, J.D. (1990) Fungal infections in bone marrow transplant patients. Semin. Oncol. 17, 10–13.
Walsh, T.J., Heir, D.B. and Caplan, I.R. (1985) Aspergillosis of central nervous system: Clinicopathological analysis of 17 patients. Ann. Neurol. 18, 574–582.
Agarwal, A.K., Bhagat, R., Panchal, N. and Shah, A. (1996) Allergic bronchopulmonary aspergillosis with aspergilloma mimicking fibrocavitary pulmonary tuberculosis. Asian Paci. J. Aller. Immunol. 14, 5–8.
Banani, B., Joshi, A.P., Sarma, P.U. and Ray, S. (1992) Evaluation of clinico-immunological parameters in pediatric ABPA patients. Ind. J. Pediatr. 59, 109–114.
Sharma, G.L. and Sarma, P.U. (1993) Luminescent immunoassay for detection of specific antibodies in allergic bronchopulmonary aspergillosis. Serodiag. Immunother. Inf. Dis. 5, 186–188.
Wang, J.L., Patterson, R., Rosenberg, M. and Cooper, B.J. (1978) Serum lgE and lgG antibody activity againstA. fumigatus as a diagnostic aid in allergic bronchopulmonary aspergillosis. Am. Rev. Respir. Dis. 117, 917–927.
de Repentigny, L. and Reiss, E. (1984) Current trends in immunodiagnosis of candidiasis and aspergillosis. Rev. Inf. Dis. 6, 301–312.
de Repentigny, L. (1989) Serological techniques for diagnosis of fungal infection. Eur. J. Clin. Microbiol. Inf. Dis. 4, 362–375.
Kauffman, H.F. and de Vries, K. (1980) Antibodies againstAspergillus fumigatus. II Identification and quantification by means of crossed immunoelectrophoresis. Int. Arch. Aller. Appl. Immunol. 62, 265–275.
Sepulveda, R., Longbottom, J.L. and Pepys, J. (1979) Enzyme-linked immunosorbent assay (ELISA) for lgG and lgE antibodies to proteins and polysaccharide antigens ofAspergillus fumigatus. Clin. Aller. 9, 359–371.
Banani, B., Mukherjee, M., Gangal, S.V. and Sarma, P.U. (1990) Studies onAspergillus fumigatus antigens for serodiagnosis. Serodiag. Immunother. Inf. Dis. 4, 183–191.
Haynes, K.A., Latge, J.P. and Rogers, T.R. (1990) Detection ofAspergillus antigens associated with invasive infection. J. Clin. Microbiol. 28, 2040–2044.
Yu, B., Niki, Y. and Armstrong, D. (1990) Use of immunoblotting to detect Aspergillus fumigatus antigen in sera and urine of rats with experimental aspergillosis. J. Clin. Microbiol. 28, 1575–1579.
Sharma, G.L., Bhatnagar, P.K., Chattopadhya, D., and Sarma, P.U. (1997) Analysis of HIV seropositive thalassemic children for antibodies specific forAspergillus fumigatus by luminescent immunoassay. J. Clin. Lab. Anal. 11, 343–345.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sharma, G.L., Sarma, P.U. Elisa based diagnostic test for Aspergillosis. Indian J Clin Biochem 12 (Suppl 1), 107–110 (1997). https://doi.org/10.1007/BF02873076
Issue Date:
DOI: https://doi.org/10.1007/BF02873076