Alteration of sulfatide synthesis in control and trembler mice during wallerian degeneration and remyelination

Abstract

Sulfatide synthesis from sulfate is much greater in the peripheral nerves of the Trembler mouse. After nerve transection, during Wallerian degeneration, this synthesis rate drops down very rapidly in both normal and Trembler mice. Twenty-four hours after permanent transection, the rate of synthesis is reduced by 80% in the mutant and 50% in the normal mouse. Four days after transection, the synthesis rate in the Trembler is only 9% of that observed in intact nerves and 21% of that in the intact nerves of normal animals. After 5 d the synthesis remains constant. Thus, enhanced synthesis of sulfatides in the Trembler mouse is probably not caused by Wallerian degeneration.

After crush of the sciatic nerve, the synthesis rate decreases very rapidly in the normal mouse as it does after permanent transection. But during regeneration, from the 7th day, it rises dramatically and 14 d after crush, a 2.5-fold increase in the synthesis rate is observed, compared to that in the contralateral control nerve. This synthesis rate returns to normal 1 mo after crush. In the Trembler, the synthesis decreases for 2 d after crush and increases from then on, eventually reaching the value of the contralateral control Trembler nerve within 2 mo. In the mutant there is no prominent peak of sulfatide synthesis during regeneration.