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Expression of Japanese quail ovalbumin inSaccharomyces cerevisiae

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Abstract

A cDNA sequence coding for Japanese quail ovalbumin was used for the construction of expression plasmid under the ADH1 promoter of the yeast shuttle vector pVT101-U. The resulting recombinant expression vector pJK2 was used for the transformation ofSaccharomyces cerevisiae. Expression of quail ovalbumin in yeast cells was demonstrated by Western blotting followed by immunochemical detection.

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Križková, L., Jeloková, J., Klaudíny, J. et al. Expression of Japanese quail ovalbumin inSaccharomyces cerevisiae . Folia Microbiol 37, 273–278 (1992). https://doi.org/10.1007/BF02814562

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