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Induced genetic deficiency of the nucleolar organizer in rat kangaroo cells (PTK1) by ultraviolet laser microirradiation

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Abstract

An ultraviolet laser microbeam was used to irradiate one of the two nucleolar organizer regions of PTK1 cells in early prophase. The directed nucleolar deficiency induced by ultraviolet laser irradiation was maintained in the daughter cells through subsequent cell generations. However, the frequent occurrence of spontaneous cell fusion in low density cells following the cloning procedure facilitated a recovery of cells to two or more nucleoli.

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References

  1. Berns, M. W., Ohnuki, Y., Rounds, D. E., Olson, R. S. (1970),Exp. Cell Res. 60, 133.

    Article  PubMed  CAS  Google Scholar 

  2. Berns, M. W., and Cheng W. K. (1971),Exp. Cell. Res. 69, 185.

    Article  PubMed  CAS  Google Scholar 

  3. Ohnuki, Y., Rounds, D. E., Olson, R. S., and Berns, M. W. (1972),Exp. Cell Res. 71, 132.

    Article  Google Scholar 

  4. Berns, M. W., Floyd, A. D., Adkisson, K., Cheng, W. K., Moore, L., Hoover, G., Ustick, K., Burgott, S., and Osial, T. (1972),Exp. Cell Res. 75, 424.

    Article  PubMed  CAS  Google Scholar 

  5. Berns, M. W. (1972),Nature 240, 483.

    Article  PubMed  CAS  Google Scholar 

  6. Berns, M. W. (1974),Int. Rev. Cytol. 39, 383.

    Article  Google Scholar 

  7. Berns, M. W. (1974),Cold Spring Harbor Symp. Quant. Biol. 38, 165.

    PubMed  CAS  Google Scholar 

  8. Berns, M. W. (1974),Science 186, 700.

    Article  PubMed  CAS  Google Scholar 

  9. Berns, M. W. (1978), inMethods in Cell Biology, vol. 18, Stein, G., Stein, J., and Kleinsmith, L., eds., Academic Press, p. 277.

  10. Berns, M. W., Chong, L. K., Hammer-Wilson, M., Miller, K., and Siemens, A. (1979),Chromosoma 73, 1.

    Article  PubMed  CAS  Google Scholar 

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Author notes

  1. Hong Liang

    Present address: Institute of Genetics, Academia Sinica, Peking, People's Republic of China

Authors and Affiliations

  1. Departmental and Cell Biology, University of California, 92717, Irvine, CA

    Hong Liang & Michael W. Berns

Authors
  1. Hong Liang
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  2. Michael W. Berns
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Liang, H., Berns, M.W. Induced genetic deficiency of the nucleolar organizer in rat kangaroo cells (PTK1) by ultraviolet laser microirradiation. Cell Biophysics 5, 21–31 (1983). https://doi.org/10.1007/BF02788584

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  • DOI: https://doi.org/10.1007/BF02788584

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