Skip to main content
SpringerLink
Log in

Preliminary report on microencapsulated islet transplantation in experimental diabetes mellitus in China

  • Practical Report
  • Published:
International journal of pancreatology Aims and scope Submit manuscript

Summary

Pancreatic islets were encapsulated with sodium alginate-polylysine after procurement by collagenase digestion and Ficoll density-gradient-dispersion. The encapsulated islets were intact and during the first week of culture produced insulin 63±42 μU/d vs 69±42 μU/d by noncapsulated islets. Seven streptozotocin-induced diabetic rats each received 4.0–4.5×103 microencapsulated islets intraperitoneally After transplantation, blood glucose dropped from 350±29 mg/dl to 142±12 mg/dl. To date, normoglycemia has been maintained for 222 days. Control animals either died or developed cataracts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Chen, J.L., Progress in endocrinllogy and metabolism. In: X.Z. Qian, J.P. Wu and X.Y. Zhu (Eds.), Annals of Chinese Medical Sciences, Science and Technology Publishers, Tian-Jin, 1984; 327–333.

    Google Scholar 

  2. Sutherland, D.E.R., Pancreas and islet transplantation, Part 1 (Experimental studies), Diabetologia, 1981; 20: 161–185.

    PubMed  CAS  Google Scholar 

  3. Lacy, P.E., Experimental immuno-alteration, World J. Surg., 1984; 8: 196–203.

    Article  Google Scholar 

  4. Lau, H., Reemtsma, K. and Hardy, M.A., Prolongation of rat islet allograft survival by direct ultraviolet irradiation of the graft, Science, 1984; 223: 607–609.

    Article  PubMed  CAS  Google Scholar 

  5. Scharp, D.W., Mason, N.S. and Sparks, R.E., Islet immuno-isolation: the use of hybrid artificial organs to prevent islet tissue rejection, World J. Surg., 1984; 8: 221–229.

    Article  PubMed  CAS  Google Scholar 

  6. Sun, A.M., O'shea, G.A. and Goosen, M.F.A., Injectable microencapsulated islet cells as a bioartificial pancreas, Appl. Biochem. Biotech., 1984; 10: 87–99.

    Article  CAS  Google Scholar 

  7. Chang, T.M.S., Artificial cells, Charles Thomas, Springfield, IL, 1972.

    Google Scholar 

  8. Gray, D.W.R., Phil, D. and Morris, P.J., Prospects for pancreatic islet transplantation, World J. Surg., 1986; 10: 410–421.

    Article  PubMed  CAS  Google Scholar 

  9. Hu, Y., The effect of islet transplant on the metabolic disorders and complications of the diabetic recipients, Chin. J. Organ Trans., 1986; 7: 50–55.

    Google Scholar 

  10. Darquy, S. and Reach, G., Immunoisolation of pancreatic B cells by microencapsulation, Diabetologia, 1985; 28: 776–780.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Surgery, Zhong Shan Hospital, Shanghai Medical University, 200032, Shanghai, P.R. China

    Zhaoguang Wu M.D. & Zhaonian Lu

  2. Department of Physiology, University of Toronto, M5S 1A8, Toronto, Ont., Canada

    Anthony M. Sun

Authors
  1. Zhaoguang Wu M.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhaonian Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Anthony M. Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wu, Z., Lu, Z. & Sun, A.M. Preliminary report on microencapsulated islet transplantation in experimental diabetes mellitus in China. Int J Pancreatol 3, 91–100 (1988). https://doi.org/10.1007/BF02788227

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02788227

Key words

Search

Navigation