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Interovarian differences in levels of cotinine, a major metabolite of nicotine, in women undergoing IVF who are exposed to cigarette smoke

  • Physiology
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Abstract

Purpose: Our purpose was to determine whether there is variation in levels of follicular fluid (FF) cotinine between the two ovaries of women undergoing IVF-ET who are exposed to cigarette smoke.

Methods: In 61 women, there were two to four determinations of FF cotinine levels for each of two follicles, one from each ovary. For each woman at test for significant difference between the means of both ovaries was done to test for interovarian variation.

Results: Thirty-seven nonsmokers, 8 passive smokers, and 16 active smokers differed greatly (P<0.0001) in mean FF cotinine levels: 13.0, 91.1, and 420.3 ng/ml, respectively. Fourteen women had significant differences, at theP=0.025 level or below, between their two ovaries. Five of them had differences significant at the 0.001 level. Even so, the correlation between the cotinine levels of the two ovaries was high.

Conclusions: Cotinine uptake between the two ovaries of a woman may differ approximately one-fourth of the time. In spite of these differences, the overall correlation between ovaries is high. The clear distinction in levels of FF cotinine among active, passive, and nonsmokers demonstrates the reliability of FF cotinine testing. Detection of cotinine in a large proportion of nonsmokers shows how pervasive nicotine is in the environment.

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References

  1. Zenzes MT: Cigarette smoking as a cause of delay in conception. Reprod Med Rev 1995;4:189–205

    Article  Google Scholar 

  2. Midgette AS, Baron J: Cigarette smoking and the risk of natural menopause. Epidemiology 1990;1:474–480

    Article  PubMed  CAS  Google Scholar 

  3. Zenzes MT, Wang P, Casper RF: Cigarette smoking may affect meiotic maturation of human oocytes. Hum Reprod 1995;10:101–105

    Google Scholar 

  4. Zenzes MT, Krishnan S, Krishnan B, Zhang RF, Casper RF: Cadmium accumulation in follicular fluid of women in in vitro fertilization-embryo transfer is higher in smokers. Fertil Steril 1995;64:599–603

    PubMed  CAS  Google Scholar 

  5. Weiss T, Eckert A: Cotinine levels in follicular fluid and serum of IVF patients: Effect on granulosa-luteal cell function in vitro. Hum Reprod 1989;4:482–485

    PubMed  CAS  Google Scholar 

  6. Rosevear SK, Holt DW, Lee TD, Ford WCL, Wardle PG, Hull MG: Smoking and decreased fertilisation rates in vitro. Lancet 1992;340:1195–1196

    Article  PubMed  CAS  Google Scholar 

  7. Sterzick K, Strehler E, De Santo M, Trumpp N, Abt M, Rosenbusch B, Schneider A: Influence of smoking on fertility in women attending an in vitro fertilization program. Fertil Steril 1996;65:810–814

    Google Scholar 

  8. Zenzes MT, Reed TE, Wang P, Klein J: Continine, a major metabolite of nicotine, is detectable in follicular fluids of passive smokers in in vitro fertilization therapy. Fertil Steril 1996;66:614–619

    Google Scholar 

  9. Benowitz NL, Kuyt F, Jacob P {jrIII}, Jones RT: Cotinine disposition and effects. Clin Pharmacol Ther 1983;34:604–611

    Article  PubMed  CAS  Google Scholar 

  10. Langone JJ, Gjika HB, Van Vunakis H: Nicotine and its metabolites. Radioimmunoassays for nicotine and continine. Biochemistry 1973;12:5025–5030

    Article  PubMed  CAS  Google Scholar 

  11. Gonen Y, Jacobson W, Casper RF: Gonadotropin suppression with oral contraceptives before in vitro fertilization. Fertil Steril 1990;53:282–287

    PubMed  CAS  Google Scholar 

  12. Zenzes MT, Reed TE, Casper RF: Effects of cigarette smoking and age on the maturation of human oocytes. Hum Reprod 1997;12:1736–1741

    Article  PubMed  CAS  Google Scholar 

  13. Laufer N, Tarlatzis BC, DeCherney AH, Masters JT, Haseltine FP, MacLusky N,et al.: Asynchrony between human cumulus-corona cell complex and oocyte maturation after human menopausal gonadotropin treatment for in vitro fertilization. Fertil Steril 1984;42:366–372

    PubMed  CAS  Google Scholar 

  14. Zenzes MT, Puy LA, Bielecki R: Immunodetection of cotinine protein in granulosa-lutein cells of women exposed to cigarette smoke. Fertil Steril 1997;68:76–82

    Article  PubMed  CAS  Google Scholar 

  15. Pirkle JL, Flegal KM, Bernert JT, Brody DJ, Etzel RA, Maurer KR: Exposure of the US population to environmental tobacco smoke: The Third National Health and Nutrition Examination Survey, 1988 to 1991. JAMA 1996;275:1233–1240

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Obstetrics and Gynaecology, University of Toronto, Toronto, Ontario, Canada

    Maria Teresa Zenzes

  2. Department of Zoology and Anthropology, University of Toronto, Toronto, Ontario, Canada

    T. Edward Reed

  3. Division of Reproductive Science, The Toronto Hospital Research Center, Room CCRW 1-813, 101 College Street, M5G 1L7, Toronto, Ontario, Canada

    Maria Teresa Zenzes

Authors
  1. Maria Teresa Zenzes
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  2. T. Edward Reed
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Zenzes, M.T., Reed, T.E. Interovarian differences in levels of cotinine, a major metabolite of nicotine, in women undergoing IVF who are exposed to cigarette smoke. J Assist Reprod Genet 15, 99–103 (1998). https://doi.org/10.1007/BF02766834

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  • DOI: https://doi.org/10.1007/BF02766834

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