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Lymphatic metastasis from tumors transplanted into the pre-irradiated footpad of the rat

Lymphogene metastasierung von in vorbestrahlte fu\ballen der ratte transplantierten tumoren

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Abstract

Background

In the literature there is some evidence that the incidence of metastases may increase after radiation treatment.

Methods

In order to investigate whether radiation-induced changes in the lymphatic drainage may alter the rate of lymph node metastasis, the center part of the left hind foot of rats was irradiated with a dose of 1 x 55 Gy before inoculation of tumor cells into the irradiated part of the footpad at different time intervals. Cells of 2 different tumor lines were employed. A rarely metastasising rhabdomyosarcoma, R-l, to look for a possible enhancement of lymphatic metastases, and a readily metastasising mammary carcinoma, Cl-2, in case of a possible decrease in the rate of lymphatic metastasis from tumors growing in pre-irradiated footpads.

Results

The incidence of regional lymph node metastasis decreased for R-l tumors growing in pre-irradiated footpads, but not for Cl-2 tumors. Furthermore, the average time required for lymph node metastasis to attain a reference volume of 100 mm3 is not significantly influenced by pre-irradiation of the footpad. No difference was observed in average times for doubling in volume of lymph node metastases originating from primary tumors in pre-irradiated footpads. Abscopal effects after footpad irradiation may cause a 50-fold increase in size of regional lymph nodes and, therefore, histological examination is essential for verification of lymph node metastases.

Conclusions

Damage to the lymphatic system to be expected in the irradiated footpad did not enhance the incidence of regional metastasis of R-1 tumors. A reduced rate of lymphatic metastasis contradicts earlier findings of enhanced lymphatic metastasis development of R-l tumors, growing in pre-irradiated gastrocnemius muscles. The influence of irradiation on regional metastasis formation seems to be “tumor bed” dependent for R-l tumors.

Zusammenfassung

Hintergrund

In der Literatur finden sich Hinweise, da\ die lokale Strahlenbehandlung eines PrimÄrtumors unter UmstÄnden die Metastasierungsrate erhöht.

Methodik

Untersucht wurde an zwei syngenen Tumormodellen der Ratte, ob durch eine Anderung der Lymphdrainage als Folge einer lokalen Vorbestrahlung des Tumorbetts eine Zunahme der lymphogenen Metastasierung verursacht wird. Zu verschiedenen Zeitpunkten nach Bestrahlung des linken Mittelfu\es mit 55 Gy Einzeldosis wurden Tumorzellen lokal injiziert. Das gering metastasierende Rhabdomyosarkom R-l sollte hierbei dem Nachweis einer eventuellen Erhöhung der Metastasierungsrate dienen, das extrem metastasierungsfreudige Mammakarzinom Cl-2 zur Erfassung einer eventuellen Erniedrigung der Metastasierungsrate.

Ergebnisse

Die HÄufigkeit regionaler Metastasen nahm beim R-1-Tumor ab, nicht jedoch beim Cl-2-Karzinom. Die durchschnittlich benötigte Zeit zum Erreichen eines Lymphknotenmetastasenvolumens von 100 mm3 war im wesentlichen von einer Vorbestrahlung des Tumorbetts unabhÄngig, ebenso die Verdopplungszeit von 100 auf 200 mm3. Als “abscopal effect” wurde eine bis zu 50fache Vergrö\erung der homolateralen poplitealen Lymphknoten nach Fu\bestrahlung ohne Tumorzellinjektion beobachtet, was auf die klinische Bedeutung einer histologischen Absicherung hinweist.

Schlu\folgerungen

Die anzunehmende lokale SchÄdigung der. LymphgefÄ\e aufgrund der Vorbestrahlung bedingte keine Erhöhung der regionalen Metastasierungsrate im Fall des R-1-Tumors. Die beobachtete Erniedrigung der Rate steht im Widerspruch mit früheren Befunden nach einer Implantation in den vorbestrahlten Gastroknemiusmuskel und deutet auf eine vom jeweiligen Tumorbett abhÄngige Strahlenwirkung.

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Authors and Affiliations

  1. Department of Radiotherapy, Reinier de Graaf Gasthuis, P.O. BOX 5011, 2600, GA Delft, The Netherlands

    Hubert J. M. van der Planken

  2. Department of Clinical Oncology, Leiden, The Netherlands

    Anton F. Hermens

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  1. Hubert J. M. van der Planken
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  2. Anton F. Hermens
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Correspondence to Hubert J. M. van der Planken.

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van der Planken, H.J.M., Hermens, A.F. Lymphatic metastasis from tumors transplanted into the pre-irradiated footpad of the rat. Strahlentherapie und Onkologie 175, 32–38 (1999). https://doi.org/10.1007/BF02743459

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  • DOI: https://doi.org/10.1007/BF02743459

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