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The quantitative analysis of multiple mRNA species using oligonucleotide probes in an S1 nuclease protection assay

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Abstract

The quantitative measurement of steady-state mRNA levels is fundamental to the analysis of gene expression. A variety of techniques are widely used to achieve this including Northern blotting, RNase protection, and S1 nuclease protection. We describe here in detail a relatively recent extension of the S1 nuclease protection technique (1) in which radiolabeled oligonucleotides are used as probes in a solution hybrieization assay (2). The principle advantage of this technique is that it allows, in a single RNA sample, the simultaneous measurement of the relative levels of at least six mRNA species, including that of a control mRNA. Further, a large number of RNA samples can be analyzed at one time.

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Author notes

  1. Thora A. Glencorse

    Present address: Institute of Biomedical and Life Sciences, Division of Molecular Genetics, University of Glasgow, Robertson Building, G11 6NU, Glasgow, UK

Authors and Affiliations

  1. Department of Pharmacology, Faculty of Medicine, University of Alberta, 9-70 Medical Sciences Building, T6G 2H7, Edmonton, Alberta, Canada

    Véronique A. -M. I. Tanay, Brian P. Tancowny, Thora A. Glencorse & Alan N. Bateson

  2. Division of Neuroscience, Faculty of Medicine, University of Alberta, 9-70 Medical Sciences Building, T6G 2H7, Edmonton, Alberta, Canada

    Véronique A. -M. I. Tanay, Brian P. Tancowny, Thora A. Glencorse & Alan N. Bateson

Authors
  1. Véronique A. -M. I. Tanay
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  2. Brian P. Tancowny
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  3. Thora A. Glencorse
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  4. Alan N. Bateson
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Correspondence to Alan N. Bateson.

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Tanay, V.A.M.I., Tancowny, B.P., Glencorse, T.A. et al. The quantitative analysis of multiple mRNA species using oligonucleotide probes in an S1 nuclease protection assay. Mol Biotechnol 7, 217–229 (1997). https://doi.org/10.1007/BF02740813

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