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Insulin-like growth factor-I (IGF-I) colocalizes with IGF-binding proteins (IGFBPs) in mouse and rat ovary

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Abstract

The cellular localization of insulin-like growth factor (IGF)-I mRNA, IGF-I peptide, and IGF-binding proteins (IGFBPs) was examined in mouse and rat ovaries through use ofin situ hybridization and immunohistochemical methods. IGF-I mRNA was found to be most abundant in granulosa cells, although lower levels were also detected in cells of the theca interna, stroma, and corpus luteum. In contrast, IGF-I immunoreactivity was undetectable or low in granulosa cells, weak and variable in oocytes, high in theca interna and the corpus luteum, and highest in the stroma. Antibodies directed against IGFBP-2, 3, and 5 yielded similar patterns of immunoreactivity to that observed for IGF-I peptide. The results indicate that IGF-I is synthesized in ovarian follicles, and that IGF-I of ovarian or systemic origin becomes localized to sites containing IGFBPs in the ovary.

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Authors and Affiliations

  1. Department of Medical Biochemistry, Faculty of Medicine, University of Calgary, 3330 Hospital Drive N.W., T2N 4N1, Calgary, Alberta, Canada

    Sabine Goubau & Gilbert A. Schultz

  2. C.R.R.A., Faculté de Médecine Vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada

    Bruce D. Murphy

  3. Department of Pediatrics, The Lawson Research Institute, University of Western Ontario, London, Ontario, Canada

    Victor K. M. Han

Authors
  1. Sabine Goubau
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  2. Bruce D. Murphy
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  3. Victor K. M. Han
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  4. Gilbert A. Schultz
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Goubau, S., Murphy, B.D., Han, V.K.M. et al. Insulin-like growth factor-I (IGF-I) colocalizes with IGF-binding proteins (IGFBPs) in mouse and rat ovary. Endocr 4, 213–221 (1996). https://doi.org/10.1007/BF02738687

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  • DOI: https://doi.org/10.1007/BF02738687

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