Abstract
Diagnostic utility of an enzyme linked immunosorbent assay (ELISA) in hospitalised patients with clinical symptoms suggestive of tuberculous meningitis (94 cases) was studied. CSF Anti-tubercular IgG antibody levels were estimated by ELISA in 44 cases of proven tuberculous meningitis, 24 proven pyogenic meningitis and in 48 non-tuberculous cases. The cut off dilution was established at a CSF dilution of 1 :1 (undiluted) with positive and negative controls. The ELISA test had a sensitivity of 97.72% and a specificity of 95.35% using MSE antigen.
When compared with conventional smear examination and culture isolation from specimens, the ELISA test proved statistically superior in the detection rate or diagnosis of clinically suspected tuberculous meningitis cases (P < 0.05).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Rao KN.Text book of Tuberculosis. New Delhi: Vikas Publishing House, 1981; 379.
Benjamin RG, Debanne SM, Ma Y and Daniel TM. Evaluation of mycobacterial antigens using an enzyme linked immunosorbent assay (ELISA) for the serodiagnosis of tuberculosis.J Med Microbiol 1984; 18: 309.
Grange JM: Mycobacterial Diseases (1)Current Topics in Infection Series 1980. London : Edward Arnold; 33.
Traunt JB, Brett WA, Thomas W Jr. Fluorescence microscopy of tubercle bacilli stained with auramine and rhodamine.Henry Ford Hosp Med Bull 1962; 10: 287–290.
Kubica GP. Differential identification of mycobacteria, VII key features for identification of clinically significant mycobacteria.Am Rev Resp Dis 1973; 107: 9–19.
Mancini G, Carbonara AO, Heremans JF. Immunochemical quantitation of antigens by single immunodiffusion.Immunochemistry 1975; 2: 235–239.
Voller A, Bidwell DE, Bartelett E. Enzyme immunoassay in diagnostic medicine theory and practise,Bull WHO 1976; 53: 55–65.
Bennedsen J. Surface Immunogens ofM. tuberculosis demonstrated by Immunofluorescence.Acta Pathol Microbiol Immunol Scand B 1970; 78 (B): 701–710.
Lowry OH, Rosenbrough NJ, Farr AL, Randal RJ. Protein measurement with folin phenol reagent.J Biol Chem 1951; 193: 265.
Udani PM, Samnel A, Kadiwal GV et al. Diagnosis of intrathoracic tuberculosis by detection of tubercle bacilli and/or tubercular antigen (TB AG) in bronchial aspirate.Indian Pediatr 1987; 54: 69–77.
Samuel AM, Kadival GV and Ashtekar MD. Serodiagnostic tests in tuberculosis.Indian J Tuberc 1985; 32: 19–28.
Maniar P.Laboratory diagnosis of disseminated and non-disseminated tuberculosis with reference to ELISA. M.Sc. Thesis, University of Bombay, Bombay, 1988; 139.
Kalish SB, Radin RC, Phair JP et al. Use of an enzyme-linked immunosorbent assay technique in the differential diagnosis of active tuberculosis in humans.J Infect Dis 1983; 147: 523–530.
Kiran U, Shriniwas, Kumar R, Sharma A. Efficacy of three mycobacterial antigens in the serodiagnosis of tuberculosis.Eur J Resp Dis 1985; 166: 187–189.
Chawla TC, Sharma A, Kiran U et al. Se rodiagnosis of intestinal tuberculosis by enzyme immunoassay and soluble antigen fluorescent antibody tests using a saline extracted antigen.Tubercle 1986; 67 ; 55–60.
Shriniwas. Advances in laboratory diagnosis of tuberculosisIndian J Med Microbiol 1984; 2: 79–94.
Samuel AM, Kadival GV, Irani S et al. A sensitive and specific method for diagnosis of tubercular meningitis.Indian J Med Res 1983; 77: 752–757.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Maniar, P., Joshi, L. ELISA-its evaluation in diagnosis of tuberculosis meningitis. Indian J Pediatr 57, 667–672 (1990). https://doi.org/10.1007/BF02728709
Issue Date:
DOI: https://doi.org/10.1007/BF02728709