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In vitro synthesis of angiotensin-converting enzyme by alveolar macrophages is increased in disseminated sarcoidosis

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Abstract

To determine the responsibility of alveolar macrophage (AM) for the increased concentration of angiotensin-converting enzyme (ACE) in bronchoalveolar fluid of patients with sarcoidosis, we cultured AM recovered by bronchoalveolar lavage of 30 subjects: 9 were normal control subjects (group C), 10 had intrathoracic localized sarcoidosis (group LS), and 11 had disseminated intrathoracic and extrathoracic sarcoidosis (group DS). Cells were cultured for 7 days and synthesis of ACE was evaluated according to the difference between final (ACEqe) and initial (ACEqi) ACE content. In groups C, LS, and DS, ACEqi were, respectively, 3.0 ± 1.7, 3.3 ± 1.9, and 4.8 ± 2.4 U/106 AM, and ACEqe were 6.2 ± 1.7, 6.7 ± 2.5, and 11.3 ± 2.8 U/106 AM. ACEqe was higher than ACEqi in all 3 groups (p < 0.01). When cycloheximide was added to the AM culture, ACEqe did not differ from ACEqi, in opposition to what was observed without cycloheximide (p < 0.002). ACEqi in group LS and DS did not differ from ACEqi in Group C, but ACEqe in Group DS was higher than in Groups C and LS (p < 0.01). We conclude that AM both from normal subjects and from subjects with sarcoidosis contain ACE, cultured AM synthesize ACE, and that a greater amount of ACE is produced when AM are obtained from patients with disseminated sarcoidosis.

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References

  1. Dobuler KJ, Catravas JD, Gillis CN (1982) Early detection of oxygen-induced lung injury in conscious rabbits. Reduced in vivo activity of angiotensin converting enzyme and removal of 5-hydroxytryptamine. Am Rev Respir Dis 126:534–539

    PubMed  CAS  Google Scholar 

  2. Friedland J, Setton C, Silverstein E (1977) Angiotensin converting enzyme: induction by steroids in rabbit alveolar macrophages in culture. Science 197:64–65

    Article  PubMed  CAS  Google Scholar 

  3. Friedland J, Setton C, Silverstein E (1978) Induction of angiotensin converting enzyme in human monocytes in cultures. Biochem Biophys Res Commun 83:843–849

    Article  PubMed  CAS  Google Scholar 

  4. Friedland J, Silverstein E (1976) A sensitive fluorimetric assay for serum angiotensin-converting enzyme. Am J Clin Pathol 66:416–424

    PubMed  CAS  Google Scholar 

  5. Gee JBL, Bodel PT, Hinman LM, Stevens CA, Matthay RA, Reynolds HY (1980) Mononuclear phagocytes in sarcoidosis: lysozyme and angiotensin I converting enzyme. In: Williams WJ, Davies BH (eds) Eight international conference on sarcoidosis and other granulomatous diseases. Alpha Omega Publishing, Cardiff, pp 417–425

    Google Scholar 

  6. Gupta RG, Sicilian L, Catchatourian R, Bekerman C, Oparil S, Szidon JP (1982) Angiotensin converting enzyme in serum and in bronchoalveolar lavage in sarcoidosis. Respiration 43:153–157

    PubMed  CAS  Google Scholar 

  7. Hayes LW, Goguen CA, Ching SF, Slakey LL (1978) Angiotensin-converting enzyme: accumulation in medium from cultured endothelial cells. Biochem Biophys Res Commun 82:1147–1153

    Article  PubMed  CAS  Google Scholar 

  8. Hinman LM, Stevens C, Matthay RA, Gee JBL (1979) Angiotensin convertase activities in human alveolar macrophages: effects of cigarette smoking and sarcoidosis. Science 205:202–203

    Article  PubMed  CAS  Google Scholar 

  9. Hollinger MA, Giri SN, Patwell S, Zuckerman JE, Gorin A, Parsons G (1980) Effect of acute lung injury on angiotensin converting in serum, lung lavage, and effusate. Am Rev Respir Dis 121:373–376

    PubMed  CAS  Google Scholar 

  10. Krieger B, Schwartz J, Loomis W, Marsh J, Spragg R (1984) Nonspecificity of elevated angiotensin converting enzyme activity in bronchoalveolar lavage fluid from high permeability lung edema states. Am Rev Respir Dis 129:499–500

    PubMed  CAS  Google Scholar 

  11. Lieberman J, Nosal A, Schlessner LA, Sastre-Foken A (1979) Serum angiotensin-converting enzyme for diagnosis and therapeutic evaluation of sarcoidosis. Am Rev Respir Dis 120:329–335

    PubMed  CAS  Google Scholar 

  12. Mordelet-Dambrine MS, Stanislas-Leguern GM, Huchon GJ, Baumann FC, Marsac JH, Chrétien J (1982) Elevation of the bronchoalveolar concentration of angiotensin I converting enzyme in sarcoidosis. Am Rev Respir Dis 126:472–475

    PubMed  CAS  Google Scholar 

  13. Nukima T, Matsuoka R, Takagi H, Ishii Y, Arai T, Kira S (1982) Responses of serum and lung angiotensin-converting enzyme activities in the early phase of pulmonary damage induced by oleic acid in dogs. Am Rev Respir Dis 126:1080–1086

    Google Scholar 

  14. Ody C, Junod AF (1977) Converting enzyme activity in endothelial cells isolated from pig pulmonary artery and aorta. Am J Physiol 232:C95-C98

    PubMed  CAS  Google Scholar 

  15. Okabe T, Suzuki A, Ishikawa H, Yotsumoto H, Ohsawa N (1981) Cells originating from sarcoid granulomas in vitro. Am Rev Respir Dis 124:608–612

    PubMed  CAS  Google Scholar 

  16. Perrin-Fayolle M, Pacheco Y, Harf R, Montagnon B, Biot N (1981) Angiotensin converting enzyme in bronchoalveolar lavage fluid in pulmonary sarcoidosis. Thorax, 34:790–792

    Article  Google Scholar 

  17. Romer FK (1979) Angiotensin-converting enzyme in sarcoidosis. Acta Med Scan 206:27–30

    CAS  Google Scholar 

  18. Roth C, Huchon GJ, Arnoux A, Stanislas-Leguern G, Marsac JH, Chrétien J (1981) Bronchoalveolar cells in advanced pulmonary sarcoidosis. Am Rev Respir Dis 124:9–12

    PubMed  CAS  Google Scholar 

  19. Ryan US, Ryan JW, Whitaker C, Chiu A (1976) Localisation of angiotensin converting enzyme (kinase II) II. Immunocytochemistry and immunofluorescence. Tissue Cell 8:125–145

    Article  PubMed  CAS  Google Scholar 

  20. Shasby DM, Shasby SS, Bowman CM, Fox RB, Harada RM, Tate RM, Repine JE (1981) Angiotensin converting enzyme concentrations in the lung lavage of normal rabbits treated with nitrogen mustard exposed to hyperoxia. Am Rev Respir Dis 124:202–203

    PubMed  CAS  Google Scholar 

  21. Silverstein E, Friedland J, Lyons HA, Gourin A (1976) Elevation of angiotensin-converting enzyme in granulomatous lymph nodes and serum sarcoidosis; clinical and possible pathogenic significances. Ann NY Acad Sci 278:498–513

    Article  PubMed  CAS  Google Scholar 

  22. Silverstein E, Friedland J, Lyons HA, Gourin A (1976) Markedly elevated antiotensin converting enzyme containing non necrotizing granuloma in granuloma. Proc Natl Acad Sci USA 73:2137–2141

    Article  PubMed  CAS  Google Scholar 

  23. Silverstein E, Friedland J, Setton C (1978) Angiotensin converting enzyme in macrophages and Freund’s adjuvant granuloma. Isr J Med Sci 14:314–318

    PubMed  CAS  Google Scholar 

  24. Silverstein E, Friedland J, Stanek AE Jr, Smith PR, Deason DR, Lyons HA (1981) Angiotensin converting enzyme: pathogenesis of sarcoidosis. Mechanisms of angiotensin converting enzyme elevation: T-lymphocyte modulation of enzyme induction in mononuclear phagocytes; enzyme properties. In: Chrétien J, Marsac J, Saltiel JC (eds) IXth international conference on sarcoidosis and other Granulomatous disorders. Pergamon Press, Paris, pp 319–325

    Google Scholar 

  25. Silverstein E, Pertshuk LP, Friedland J (1979) Immunofluorescent localization of angiotensin converting enzyme in epithelioid and giant cells of sarcoidosis granuloma. Proc Natl Acad Sci USA 76:6646–6648

    Article  PubMed  CAS  Google Scholar 

  26. Stanislas-Leguern G, Choudat D, Arnoux A, Durieux P, Lacronique J, Baumann FC, Huchon G, Marsac J, Chrétrien J (1981) Pronostic value of angiotensin converting enzyme and elveolar lymphocytes in sarcoidosis. In: Chrétien J, Marsac J, Saltiel JC (eds) IXth international conference on sarcoidosis and other granulomatous disorders. Pergamon Press, Paris, pp 424–429

    Google Scholar 

  27. Stanislas-Leguern G, Leclerc P, Baumann FC, Mordelet-Dambrine M, Huchon GJ, Andreux JP, Marsac J, Rochemaure J, Chrétrien J (1981) Angiotensin converting enzyme in bronchoalveolar fluid and serum as indicators of spread of untreated sarcoidosis. In: Chrétien J, Marsac J, Saltiel JC (eds) IXth international conference on sarcoidosis and other granulomatous disorders. Pergamon Press, Paris, pp 356–361

    Google Scholar 

  28. Studdy P, Bird R, James DG, Sherlock S (1978) Serum angiotensin-converting enzyme (SACE) in sarcoidosis and other granulomatous disorders. Lancet 2:1331–1334

    Article  PubMed  CAS  Google Scholar 

  29. Winer BJ (1971) Statistical principles in experimental design. McGraw-Hill, New-York

    Google Scholar 

  30. Zar JH (1974) Biostatistical analysis. Prentice Hall, Englewood Cliffs, NJ

    Google Scholar 

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Authors and Affiliations

  1. Hôpital Laennec, Université René-Descartes, Inserm U 214, Paris, France

    Gisèle Stanislas-Leguern, Madeleine Mordelet-Dambrine, Daniel Dusser, Michèle Huesca, Jacques Chretien & Gérard J. Huchon

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  1. Gisèle Stanislas-Leguern
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  2. Madeleine Mordelet-Dambrine
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  3. Daniel Dusser
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  4. Michèle Huesca
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  5. Jacques Chretien
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  6. Gérard J. Huchon
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Stanislas-Leguern, G., Mordelet-Dambrine, M., Dusser, D. et al. In vitro synthesis of angiotensin-converting enzyme by alveolar macrophages is increased in disseminated sarcoidosis. Lung 164, 269–277 (1986). https://doi.org/10.1007/BF02713651

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