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A comparative phylogenetic analysis of full-lengthmariner elements isolated from the Indian tasar silkmoth,Antheraea mylitta (Lepidoptera: saturniidae)

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Abstract

Mariner like elements (MLEs) are widely distributed type II transposons with an open reading frame (ORF) for transposase. We studied comparative phylogenetic evolution and inverted terminal repeat (ITR) conservation of MLEs from Indian saturniid silkmoth,Antheraea mylitta with other full length MLEs submitted in the database. Full length elements fromA. mylitta were inactive with multiple mutations. Many conserved amino acid blocks were identified after aligning transposase sequences. Mariner signature sequence, DD(34)D was almost invariable although a few new class of elements had different signatures.A. mylitta MLEs(Anmmar) get phylogenetically classified under cecropia subfamily and cluster closely with the elements from other Bombycoidea superfamily members implying vertical transmission from a common ancestor. ITR analysis showed a conserved sequence of AGGT(2-8N)ATAAGT for forward repeat and AGGT(2-8N)ATGAAAT for reverse repeat. These results and additional work may help us to understand the dynamics of MLE distribution inA. mylitta and construction of appropriate vectors for mariner mediated transgenics.

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Abbreviations

ITRs:

Inverted terminal repeats

MLE:

mariner like elements

ORF:

open reading frame

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Authors and Affiliations

  1. Laboratory of Molecular Genetics, Center for DNA Fingerprinting and Diagnostics (CDFD), ECIL Road, Nacharam, 500 076, Hyderabad, India

    M. Dharma Prasad & J. Nagaraju

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  1. M. Dharma Prasad
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  2. J. Nagaraju
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Correspondence to J. Nagaraju.

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Prasad, M.D., Nagaraju, J. A comparative phylogenetic analysis of full-lengthmariner elements isolated from the Indian tasar silkmoth,Antheraea mylitta (Lepidoptera: saturniidae). J Biosci 28, 443–453 (2003). https://doi.org/10.1007/BF02705119

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  • DOI: https://doi.org/10.1007/BF02705119

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