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Preparation of β-cyclodextrin sulfate-immobilized hydrophilic vinyl-polymer gel as a selective, high recovery and stable adsorbent for high-performance affinity chromatography of heparin-binding substances

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Summary

β-Cyclodextrin sulfate having heparin-mimicking activity was immobilized on a hydrophilic vinyl-polymer gel, TSKgel AF-Epoxy, TOYOPEARL 650M. A column packed with this material could be used for high-performance affinity chromatographic separation of heparin-binding substances such as growth factors, enzymes, coagulation factors and lipoproteins. The substances retained on the column were recovered more easily than those on a heparin-immobilized column. Furthermore, the β-Cyclodextin sulfate-immobilized gel was superior in stability to the heparin-immobilized gel when exposed to acidic and basic solutions.

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Authors and Affiliations

  1. Department of Drug Formulations, Kaken Pharmaceutical Co. Ltd., 301 Gensuke, Fujieda-Shi, 426, Shizuoka, Japan

    K. Ishimura & K. Fukunaga

  2. Faculty of Pharmaceutical Sciences, Science University of Tokyo, 12 Ichigaya-Funagawara-Machi, Shinjuku-ku, 162, Tokyo, Japan

    T. Ohta & H. Nakamura

  3. Faculty of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, 862, Kumamoto, Japan

    T. Irie & K. Uekama

Authors
  1. K. Ishimura
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  2. K. Fukunaga
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  3. T. Ohta
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  4. H. Nakamura
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  5. T. Irie
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  6. K. Uekama
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Ishimura, K., Fukunaga, K., Ohta, T. et al. Preparation of β-cyclodextrin sulfate-immobilized hydrophilic vinyl-polymer gel as a selective, high recovery and stable adsorbent for high-performance affinity chromatography of heparin-binding substances. Chromatographia 41, 349–352 (1995). https://doi.org/10.1007/BF02688050

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  • DOI: https://doi.org/10.1007/BF02688050

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