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Growth and morphology of neuronal cell lines cultured in perfusion

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Summary

To optimize culture conditions and gain a more reliable culturing system for studies of metabolic properties of neuronal cells, a simplified perfusion chamber was developed. It consists of two parts: a perfusion block and a standard plastic culture dish. To confirm the suitability of this chamber for continuous culturing of anchorage-dependent cells, the growth and morphology of the four neuronal cell lines glioma C6 and glioma 138MG, neuroblastoma C1300, clones N1E115 and N18 were followed for 4 d using both traditional and perfusion techniques. A marked increase in growth and a decrease in the degree of morphological differentiation were obtained with the latter technique compared to the former.

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Authors and Affiliations

  1. Unit of Neurochemistry and Neurotoxicology, University of Stockholm, Enköpingsvägen 126, S-172 46, Sundbyberg, Sweden

    Anders Peterson & Erik Walum

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  1. Anders Peterson
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  2. Erik Walum
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Additional information

This work was supported by grants from the National Swedish Board for Technical Development (Grant 81-5009), the Swedish Work Environmental Foundation (Grant 76-53), and Ollie and Elof Ericssons Foundation for Scientific Research.

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Peterson, A., Walum, E. Growth and morphology of neuronal cell lines cultured in perfusion. In Vitro 19, 875–880 (1983). https://doi.org/10.1007/BF02661707

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  • DOI: https://doi.org/10.1007/BF02661707

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