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Intrinsic glycosylation potentials of insect cell cultures and insect larvae

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Summary

The glycosylation and subsequent processing of native and recombinant glycoproteins expressed in established insect cell lines and insect larvae were compared. TheSpodoptera frugiperda (Sf21) andTrichoplusia ni (TN-368 and BTI-Tn-5B1-4) cell lines possessed several intrinsic glycoproteins that are modified with both N- and O-linked oligosaccharides. The N-linked oligosaccharides were identified as both the simple (high mannose) and complex (containing sialic acid) types. Similarly, theT. ni larvae also possessed intrinsic glycoproteins that were modified with O-linked and simple and complex N-linked oligosaccharides. Additionally, human placental, secreted alkaline phosphatase (SEAP) produced during replication of a recombinant baculovirus inT. ni larvae was modified with complex oligosaccharide having sialic acid linked α(2–6) to galactose.

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  1. Boyce Thompson Institute for Plant Research, Tower Road, 14853, Ithaca, New York

    Thomas R. Davis & H. A. Wood

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  1. Thomas R. Davis
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Davis, T.R., Wood, H.A. Intrinsic glycosylation potentials of insect cell cultures and insect larvae. In Vitro Cell Dev Biol - Animal 31, 659–663 (1995). https://doi.org/10.1007/BF02634086

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