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A melinex coverslip method for ultrastructural studies of monolayer cultures

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Summary

A flat-face embedding method which facilitates both light microscopic and electron microscopic observations of monolayer cultures has been described. The method involves the use of an industrial plastic “Melinex” as the substratum for Leighton tube cultures. The growth patterns of rat embryo muscle cultures and muscle tumor cultures on Melinex coverslips have been shown to be comparable to those of corresponding cultures grown on glass coverslips. Melinex sheets, which may easily be substituted as substratum for any stationary culture, offer the added advantage of preserving the undisrupted architecture of monolayer cultures for electron microscopic studies.

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References

  1. Firket, H. 1966. Polyester sheeting, a tissue-culture support easily separable from epoxy resins after flat face embedding. Stain Tech. 41: 189–191.

    CAS  Google Scholar 

  2. Basrur, P. K., and J. P. W. Gilman. 1963. Behaviour of two cell strains derived from rat rhabdomyosarcomas. J. Nat. Cancer Inst. 30: 163–201.

    PubMed  CAS  Google Scholar 

  3. Swierenga, S. H. H., and P. K. Basrur. 1968. Effect of nickel on cultured rat embryo muscle cells. Lab. Invest. 19: 663–674.

    PubMed  CAS  Google Scholar 

  4. Merchant, D. J., D. J. Kahn, and W. H. Murphy. 1964.Handbook of Cell and Organ Culture, 2nd Ed., Burgess Publishing Co., Minneapolis, Minn.

    Google Scholar 

  5. Sabatini, D. D., K. Bensch and R. J. Barrnett. 1963. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 17: 19–58.

    Article  PubMed  CAS  Google Scholar 

  6. Millonig, G. 1962. Further observations on a phosphate buffer of osmium solution in fixation. In:Electron Microscopy, Fifth Intern. Congress Philadelphia, Volume 2. Academic Press, New York, p. 8.

    Google Scholar 

  7. Luft, J. H. 1961. Improvements in epoxy resin embedding resins. J. Biophys. Biochem. Cytol. 9: 409–414.

    PubMed  CAS  Google Scholar 

  8. Venable, J. H., and R. Coggeshall. 1965. A simplified lead citrate strain for use in electron microscopy. J. Cell Biol. 25: 407–408.

    Article  PubMed  CAS  Google Scholar 

  9. Holtzer, H., J. Abbott, and J. Lash. 1958. On the formation of multinucleated myotubes. Anat. Rec. 131: 567.

    Article  Google Scholar 

  10. Capers, C. R. 1960. Multinucleation of skeletal musclein vitro. J. Biophys. Biochem. Cytol. 7: 559–566.

    Article  PubMed  CAS  Google Scholar 

  11. Yaffe, D. 1969. Cellular aspects of muscle differentiation in vitro. In: Moscona, A. A., and A. Monroy (Ed.),Current Topics in Developmental Biology, Volume 4. Academic Press, New York, p. 37–77.

    Google Scholar 

  12. Paul, J. 1965. Carbohydrate and energy metabolism. In: E. N. Willmer (ed.),Cells and Tissue in Culture. Methods, Biology and Physiology, Volume 1. Academic Press, New York, p. 239–276.

    Google Scholar 

  13. Howatson, A. F., and L. D. Almeida. 1957. A method for the study of cultured cells by thin sectioning and electron microscopy. J. Biophys. Biochem. Cytol 4: 115–118.

    Google Scholar 

  14. Heyner, S. 1963.In situ embedding of cultured cells or tissue, grown on glass, in epoxy resin for electron microscopy. Stain Tech. 38: 335–336.

    CAS  Google Scholar 

  15. Flaxman, B. A., M. A. Lutzner, and E. J. Van Scott, 1968. Ultrastructure of cell attachment of substratumin vitro. J. Cell Biol. 36: 406–410.

    Article  PubMed  CAS  Google Scholar 

  16. Kushida, H., and K. Suzuki 1968. Evaporated silica as a stripping agent for coverslip-cultured cells embedded in epoxy resins. J. Electron Microsc. 17: 350–352.

    Google Scholar 

  17. Davies, C., and R. Wallace. 1958. The use of carbon films in the culture of tissue cells for electron microscopy. J. Biophys. Cytol. 4: 231–232.

    PubMed  CAS  Google Scholar 

  18. Brinkley, B. R., P. Murphy, and L. C. Richardson. 1967. Procedure for embeddingin situ selected cells culturedin vitro. J. Cell Biol. 35: 279–283.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biomedical Sciences, University of Guelph, Guelph, Ontario, Canada

    Anthony K. Sykes & Parvathi K. Basrur

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  1. Anthony K. Sykes
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  2. Parvathi K. Basrur
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This investigation was supported in part by a grant in aid of research from the National Cancer Institute of Canada.

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Sykes, A.K., Basrur, P.K. A melinex coverslip method for ultrastructural studies of monolayer cultures. In Vitro 7, 68–73 (1971). https://doi.org/10.1007/BF02628264

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