Summary
RPMI 1788 lymphocytes growtn in semi-suspension culture proliferate as separate cells and in clumps. The addition of 10−3 m dibutyryl cyclic AMP (Bu2cAMP) to the culture resulted in the attachment of the cells to the substratum and a subsequent conversion of a portion of the adherent cells to a fibroblast-like morphology. Growth of the adherent cells proceeded at nearly the same rate as that of the control cells. When the cells cultured in the presence of Bu2cAMP were periodically disturbed, they remained in suspension and under this condition a distinct inhibition of growth by Bu2cAMP was observed.
Cortisol at 10−5 m, a concentration having no effect on the proliferation of RPMI 1788 cells, when added to cells cultured in the presence of Bu2cAMP, prevented cell attachment, caused detachment of already adherent cells and thereby brought about the Bu2cAMP-mediated inhibition of growth in suspension. At a higher concentration (10−4 m), cortisol alone reduced the growth rate of RPMI 1788 lymphocytes. Under the combined effects of 10−4 m cortisol and 10−3 m Bu2cAMP, the proliferation and viability of cells in suspension were significantly lower than in the presence of either agent alone.
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Smith, S.W., Werthamer, S. & Artman, M. Differential effects of dibutyryl cyclic AMP on the growth and morphology of an established human lymphocyte line. In Vitro 10, 225–229 (1974). https://doi.org/10.1007/BF02615236
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DOI: https://doi.org/10.1007/BF02615236