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Evidence for plasmid-associated lactose metabolism inLactobacillus casei subsp.casei

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Abstract

Lac variants ofLactobacillus casei subsp.casei DR1002 (formerly 64H) have been produced using acriflavin, ethidium bromide, mitomycin C, or combinations of these agents. Two successive transfers in the presence of acriflavin and mitomycin C or ethidium bromide and mitomycin C resulted in nearly a 100% loss of lactose fermentation. Cesium chloride-ethidium bromide isopycnic gradient ultracentrifugal analysis of purified lysates demonstrated that the 23-mdal plasmid (pDR101) found inL. casei DR1002 was consistently absent in Lac− clones. We concluded that, as in lactic streptococci, lactose metablism is a plasmid-mediated train inL. casei DR1002.

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Authors and Affiliations

  1. Microbiology Section, Laboratory of Microbiology and Immunology, National Institute of Dental Research, National Institutes of Health, 20014, Bethesda, Maryland, USA

    Bruce M. Chassy, Evelyn M. Gibson & Alfred Guiffrida

Authors
  1. Bruce M. Chassy
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  2. Evelyn M. Gibson
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  3. Alfred Guiffrida
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Chassy, B.M., Gibson, E.M. & Guiffrida, A. Evidence for plasmid-associated lactose metabolism inLactobacillus casei subsp.casei . Current Microbiology 1, 141–144 (1978). https://doi.org/10.1007/BF02601666

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