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Gastrin stimulates gastric mast cells in rabbits

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Research in Experimental Medicine

Abstract

In a previous study we demonstrated that in human gastric mucosa tryptase was localized only in mast cells and that its levels were correlated with serum gastrin, suggesting a link between gastrin action and mucosal mast cell function. The aim of the present study was to discover whether pentagastrin injection could stimulate gastric mucosal mast cells in rabbits. Ten female rabbits (group S) were injected s.c. with pentagastrin (10 μg/kg); another group of ten animals (group C) was injected s.c. with an equal volume of saline solution. One hour after the injection the rabbits were sacrificed and their stomachs removed. Antrum (A), corpus (C) and fundus (F) mucosal homogenates were assayed for total protein, tryptase, pepsinogen A (PGA), histamine and gastrin. Histamine tissue levels were significantly lower in group S than in group C in the antrum (Mann-Whitney test: U=82,P<0.01) and in the corpus (U=83,P<0.005). Tryptase levels were significantly higher in group S than in group C in all gastric areas (antrum: U=95,P<0.001; corpus: U=85,P<0.005 and fundus: U=75,P<0.05). Total protein PGA and gastrin did not vary significantly between groups. In group C, no signficant correlations were found among the five parameters. In group S, corpus tryptase was correlated with fundus tryptase (Spearman'sr=0.831,P<0.01). The same relationship was observed for histamine (r=0.672,P<0.05). In group S, antrum gastrin was inversely correlated with antrum tryptase (r=0.903,P<0.001), and with corpus PGA (r=−0.806,P<0.05). This study demonstrates that bolus pentagastrin administration stimulates gastric mucosal mast cells in the rabbit.

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References

  1. Bechi P, Amorosi A, Mazzanti A, Dei R, Bianchi S, Mugnai L, Masini E (1993) Reflux-related gastric mucosal injury is associated with increased mucosal histamine content in humans. Gastroenterology 104:1057–1063

    PubMed  CAS  Google Scholar 

  2. Bechi P, Dei R, Di Bello MG, Masini E (1993) Helicobacter pylori potentiates histamine release from serosal rat mast cells in vitro. Dig Dis Sci 5:944–949

    Article  Google Scholar 

  3. Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  4. Charalabos P, Fanny K, Kelly CP, Eliakim R, Joshi MA, O'Keane CJ, Castagliuolo I, La-Mont JT, Rachmilewitz D (1993) Ketotifen inhibitsClostridium difficile toxin A-induced enteritis in rat ileum. Gastroenterology 105:701–707

    Google Scholar 

  5. Coupe M, Rees H, Springer CJ, Bishop AE, Morris JA, Polak JM, Calam J (1990) Gastric enterochromaffin-like (ECL) cells in hypergastrinemic duodenal ulcer disease. Gut 31:144–147

    PubMed  CAS  Google Scholar 

  6. Courillon-Mallet A, Callebert J, Roucayrol AM, Costil V, Launay JM, Cattan D (1992) Argyrophil cells, mast cells, and histamine in the fundic mucosa of antrectomized patients. Scand J Gastroenterol 27:656–660

    PubMed  CAS  Google Scholar 

  7. Ehrenreich H, Burd PR, Rottem M, Hultnor, L, Hylton JB, Garfield M, Coligan JE, Metcalf DD, Fauci AS (1992). Endothelins belong to the assorment of mast cell-derived and mast cell-bound cytokines. New Biol 4:147–156

    PubMed  CAS  Google Scholar 

  8. Fox CC, Lichtenstein LM, Roche JK (1993) Intestinal mast cell responses in idiopathic inflammatory bowel disease. Histamine release from human intestinal mast cells in response to gut epithelial proteins. Dig Dis Sci 38:1105–1112

    Article  PubMed  CAS  Google Scholar 

  9. Hakanson R, Sundler F (1991) Histamine-producing cells in the stomach and their role in the regulation of acid secretion. Scand J Gastroenterol 26 [Suppl 180]:88–94

    Google Scholar 

  10. Hakanson R, Bottcher G, Ekblad E, Panula P, Simonsson M, Dohlsten M, Hallberg T, Sundler F (1986) Histamine in endocrine cells in the stomach. A survey of several species using a panel of histamine antibodies. Histochemistry 86:5–17

    Article  PubMed  CAS  Google Scholar 

  11. Hogaboam CM, Bissonette EY, Beth CC, Befus AD, Wallace JL (1993) Prostaglandins inhibit inflammatory mediator release from rat mast cells. Gastroenterology 104:122–129

    PubMed  CAS  Google Scholar 

  12. Irani AMA, Craig SS, DeBlois G, Elson CO, Schechter NM, Schwartz LB (1987) Deficiency of the tryptase-positive, chymase-negative mast cell type in gastrointestinal mucosa of patients with defective T lymphocyte function. J Immunol 138:4381–4386

    PubMed  CAS  Google Scholar 

  13. Marshall JS (1993) Repeated antigen challenge in rats induces a mucosal mast cell hyperplasia. Gastroenterology 105:391–398

    PubMed  CAS  Google Scholar 

  14. Obrink KJ (1991) Histamine and gastric acid secretion. Scand J Gastroenterol 26 [Suppl 180]:4–8

    Google Scholar 

  15. Plebani M (1993) Pepsinogens in health and disease. Clin Lab Sci 30:273–328

    CAS  Google Scholar 

  16. Plebani M, Basso D, Busatto G, Di Mario F, Del Giudice G, Vianello F, Baffa R, Battistel M, Rugge M (1994) Are tryptase and cathepsin D related toHelicobacter pylori infection and mucosal gastrin in peptic ulcer? Res Exp Med 194:1–8

    CAS  Google Scholar 

  17. Roche S, Bali J-P, Galleyrand J-C, Magous R (1991) Characterization of a gastrin-type receptor on rabbit gastric parietal cells using L365,260 and L364,718. Am J Physiol 260:G182-G188

    PubMed  CAS  Google Scholar 

  18. Ryberg B, Tielemans Y, Axelson J, Carlsson E, Hakanson R, Mattson H, Sundler F, Willems G (1990) Gastrin stimulates the self-replication rate of enterochromaffin-like cell in the rat stomach. Gastroenterology 99:935–942

    PubMed  CAS  Google Scholar 

  19. Schwartz LB (1990) Tryptase for human mast cells: biochemistry, biology and clinical utility. In: Schwartz LB (ed) Neutral proteases of mast cells. (Monogr Allergy 27), Karger, Basel, pp 90–113

    Google Scholar 

  20. Schwartz LB, Irani AMA, Roller K, Castells MC, Schechter NM (1987) Quantitation of histamine, tryptase, and chymase in dispersed human T and TC mast cells. J Immunol 138:2611–2615

    PubMed  CAS  Google Scholar 

  21. Stechschulte DJ, Morris DC, Jilka RL, Stechschulte DJ, Dileepan KN (1990) (1990) Impaired gastric acid secretion in mast cell-deficient mice. Am J Physiol 259:G41-G47

    PubMed  CAS  Google Scholar 

  22. Waldum HL, Sandvik AK, Brenna E, Peterson H (1991) Gastrin-histamine sequence in the regulation of gastric acid secretion. Gut 32:698–701

    PubMed  CAS  Google Scholar 

  23. Whittle BJR (1993) Modulation by prostanoids of the release of inflammatory mediators from mast cells: involvement in mucosal protection? Gastroenterology 104:314–316

    PubMed  CAS  Google Scholar 

  24. Whittle BJR, Esplugues JV (1988) Induction of rat gastric damage by the endothelium-derived peptide, endothelin. Br J Pharmacol 95:1011–1013

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Laboratory Medicine, University of Padua, Padua, Italy

    M. Plebani, D. Basso, L. Brigato & M. Battistel

  2. Department of Histochemical and Immunohistochemical Pathology, University of Padua, Padua, Italy

    G. Busatto & M. Rugge

Authors
  1. M. Plebani
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  2. D. Basso
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  3. G. Busatto
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  4. L. Brigato
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  5. M. Battistel
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  6. M. Rugge
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Plebani, M., Basso, D., Busatto, G. et al. Gastrin stimulates gastric mast cells in rabbits. Res. Exp. Med. 195, 365–371 (1995). https://doi.org/10.1007/BF02576807

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  • DOI: https://doi.org/10.1007/BF02576807

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