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Pitfalls in pit measurement

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References

  1. Reynolds JJ, Dingle JT (1970) A sensitive in vitro method for studying the induction and inhibition of bone resorption. Calcif Tissue Res 4:339–349

    Article  PubMed  CAS  Google Scholar 

  2. Stern PH, Krieger NS (1983) Comparison of fetal rat limb bones and neonatal mouse calvaria: effects of parathyroid hormone and 1,25-Di-hydroxy Vitamin D3. Calcif Tiss Int 35:172–176

    Article  CAS  Google Scholar 

  3. Raisz LG, Alander C, Eilon G, Whitehead SP, Nuki K (1982) Effects of two bacterial products, muramyl dipeptide and endotoxin, on bone resorption in organ culture. Calcif Tiss Int 34:365–369

    Article  CAS  Google Scholar 

  4. Raisz LG, Bergmann PJ, Dominguez JH, Price MA (1979) Enhancement of parathyroid hormone-stimulated bone resorption by poly-l-lysine. Endocrinology 105:152–155

    PubMed  CAS  Google Scholar 

  5. Boyde A, Ali NN, Jones SJ (1983) Computer-aided measurement of resorptive activity of isolated osteoclasts. Proc roy microsc Soc 18:357

    Google Scholar 

  6. Boyde A, Ali NN, Jones SJ (1984) Resorption of dentine by isolated osteoclasts in vitro. Brit dent J 156:216–220

    Article  PubMed  CAS  Google Scholar 

  7. Athanasou NA, Gray A, Revell PA, Fuller K, Cochrane T, Chambers TJ (1984) Stereophotogrammetric observations on bone resorption by isolated rabbit osteoclasts. Micron and Microscopica Acta 15:47–53

    Article  Google Scholar 

  8. Chambers TJ, Revell PA, Fuller K, Athanasou NA (1984) Resorption of bone by isolated rabbit osteoclasts. J Cell Sci 66:383–399

    PubMed  CAS  Google Scholar 

  9. Boyde A, Jones SJ (1985) Optical and scanning electron microscopy in the single osteoclast resorption assay. Scanning Electron Microscopy 1985/3:1259–1271

    Google Scholar 

  10. Jones SJ, Boyde A, Ali NN, Maconnachie E (1985) A review of bone cell and substratum interactions. Scanning 7:5–24

    Google Scholar 

  11. Jones SJ, Boyde A, Ali NN, Maconnachie E (1986) Variation in the sizes of resorption lacunae made in vitro. Scanning Electron Microscopy 1986/4:1571–1580

    Google Scholar 

  12. Delaisse JM, Boyde A, Maconnachie E, Ali NN, Sear CHJ, Eeckhout Y, Vaes G, Jones SJ (1987) The effect of inhibitors of cysteine-proteinases and collagenase on the resorptive activity of isolated osteoclasts. Bone 8:305–313

    Article  PubMed  CAS  Google Scholar 

  13. Jones SJ, Boyde A (1988) The resorption of dentine and cementum in vivo and in vitro. In: Z. Davidovitch (ed) The Biological Mechanisms of Tooth Eruption and Root Resorption. EBSCO Media, Birmingham AL, pp 335–354

    Google Scholar 

  14. Arnett TR, Dempster DW (1986) Effect of pH on bone resorption by rat osteoclasts in vitro. Endocrinology 119:119–124

    PubMed  CAS  Google Scholar 

  15. Arnett TR, Dempster DW (1987) A comparative study of disaggregated chick and rat osteoclasts in vitro: effects of calcitonin and prostaglandins. Endocrinology 120:602–607

    Article  PubMed  CAS  Google Scholar 

  16. Horton MA, Taylor ML, Arnett TR, Helfrich MH (1991) Arg-Gly-Asp (RGD) peptides and the anti-vitronectin receptor antibody 23C6 inhibit dentine resorption and cell spreading by chick osteoclasts. Exp. Cell Res. (in press)

  17. Barnicot NA (1946) The supravital staining of osteoclasts with neutral-red: their distribution on the parietal bone of normal growing mice and a comparison with the mutants grey-lethal and hydrocephalus-3. Proc roy Soc B 134:467–485

    Article  Google Scholar 

  18. Zambonin-Zallone A, Teti A, Carano A, Marchisio PC (1988) The distribution of podosomes in osteoclasts cultured on bone laminae: effect of retinol. J Bone Min Res 3:517–523

    CAS  Google Scholar 

  19. Turksen K, Kanehisa J, Opas M, Heersche JNM, Aubin JE (1988) Adhesion patterns and cytoskeleton of rabbit osteoclasts on bone slices and glass. J Bone Min Res 3:389–400

    CAS  Google Scholar 

  20. Taylor ML, Boyde A, Jones SJ (1989) The effect of fluoride on the patterns of adherence of osteoclasts cultured on and resorbing dentine: a 3-D assessment of vinculin-labelled cells using confocal optical microscopy. Anat Embryol 180:427–435

    Article  PubMed  CAS  Google Scholar 

  21. Chambers TJ, Darby JA, Fuller K (1985) Mammalian collagenase predisposes bone surfaces to osteoclastic resorption. Cell Tissue Res 241:671–675

    Article  PubMed  CAS  Google Scholar 

  22. Chambers TJ, Fuller K (1985) Bone cells predispose bone surfaces to resorption by exposure of mineral to osteoclastic contact. J Cell Sci 76:155–165

    PubMed  CAS  Google Scholar 

  23. Murrills RJ, Shane E, Lindsay R, Dempster DW (1989) Bone resorption by isolated human osteoclasts in vitro: effects of calcitonin. J Bone Min Res 4:259–269

    CAS  Google Scholar 

  24. Chambers TJ, Fuller K, Darby JA, Pringle JAS, Horton MA (1986) Monoclonal antibodies against osteoclasts inhibit bone resorption in vitro. Bone and Mineral 1:127–135

    PubMed  CAS  Google Scholar 

  25. Chambers TJ, McSheehy PMJ, Thomson BM, Fuller K (1985) The effect of calcium-regulating hormones and prostaglandins on bone resorption by osteoclasts disaggregated from neonatal rabbit bones. Endocrinology 116:234–239

    Article  PubMed  CAS  Google Scholar 

  26. Chambers TJ, Fuller K, McSheehy PMJ (1985) the effects of calcium regulating hormones on bone resorption by isolated human osteoclastoma cells. J Pathology 145:297–305

    Article  CAS  Google Scholar 

  27. Chambers TJ, Thomson BM, Fuller K (1984) Effect of substrate composition on bone resorption by rabbit osteoclasts. J Cell Sci 70:61–71

    PubMed  CAS  Google Scholar 

  28. Chambers TJ, Fuller K, Darby JA (1987) Hormonal regulation of acid phosphatase release by osteoclasts disaggregated from neonatal rat bone. J Cell Physiol 132:90–96

    Article  PubMed  CAS  Google Scholar 

  29. Thomson B, Mundy GR, Chambers TJ (1987) Tumor necrosis factors alpha and beta induce osteoblastic cells to stimulate osteoclastic bone resorption. J Immunol 138:775–779

    PubMed  CAS  Google Scholar 

  30. Thomson BM, Saklatvala J, Chambers TJ (1986) Osteoblasts mediate interleukin 1 stimulation of bone resorption by rat osteoclasts. J Exp Med 164:104–112

    Article  PubMed  CAS  Google Scholar 

  31. McSheehy PMJ, Chambers TJ (1986) Osteoblast-like cells in the presence of parathyroid hormone release soluble factor that stimulates osteoclastic bone resorption. Endocrinology 119:1654–1659

    Article  PubMed  CAS  Google Scholar 

  32. McSheehy PMJ, Chambers TJ (1987) 1,25-Dihydroxyvitamin D3 stimulates rat osteoblastic cells to release a soluble factor that increases osteoclastic bone resorption. J Clin Invest 80:425–429

    Article  PubMed  CAS  Google Scholar 

  33. Hall TJ, Chambers TJ (1989) Optimal bone resorption by isolated rat osteoclasts requires chloride/bicarbonate exchange. Calcif Tissue Int 45:378–380

    Article  PubMed  CAS  Google Scholar 

  34. Fuller K, Chambers TJ (1989) Effect of arachidonic acid metabolites on bone resorption by isolated rat osteoclasts. J Bone Min Res 4:209–214

    CAS  Google Scholar 

  35. Jones SJ, Boyde A, Ali NN (1984) The resorption of biological and non-biological substrates by cultured avian and mammalian osteoclasts. Anat Embryol 170:247–256

    Article  PubMed  CAS  Google Scholar 

  36. Jones SJ, Boyde A, Ali NN (1986) The interface of cells and their matrices in mineralised tissue. Scanning Electron Microscopy 1986;4:1555–1569

    Google Scholar 

  37. Jones SJ, Ali NN, Boyde A (1986) Survival and resorptive activity of chick osteoclasts in culture. Anat Embryol 174:265–275

    Article  PubMed  CAS  Google Scholar 

  38. Reid SA (1986) Effect of mineral content of human bone on in vitro resorption. Anat Embryol 174:225–234

    Article  PubMed  CAS  Google Scholar 

  39. Taylor ML, Maconnachie E, Frank K, Boyde A, Jones SJ (1990) The effect of fluoride on the resorption of dentine by osteoclasts in vitro. J Bone Mineral Res 5 Suppl 1:S121-S130

    CAS  Google Scholar 

  40. Boyde A, Maconnachie E, Taylor ML, Jones SJ (1991) Measurement of resorption pit width and depth to assay osteoclastic function in vitro. J Anat (in press)

  41. Sato M, Grasser W (1990) Effects of bisphosphonates on isolated rat osteoclasts as examined by reflected light microscopy. J Bone Min Res 5:31–40

    CAS  Google Scholar 

  42. Berry L, Beresford JL, Boothroyd B, Evans JC, Gallagher JA (1988) Bone resorption in co-cultures of osteoclasts and bone tumour cells, assessed by reflected light microscopy and measurement of calcium release Calcif Tiss Int 42 Suppl: A42 Abst 161.

    Google Scholar 

  43. Boyde A (1973) Quantitative photogrammetric analysis and qualitative stereoscopic analysis of SEM images. J Microsc 98:452–471

    Google Scholar 

  44. Boyde A (1975) Photogrammetry and the scanning electron microscope. Photogrammetric Record 8:408–457

    Article  Google Scholar 

  45. Boyde A, Jones SJ (1979) Estimation of the size of resorption lacunae in mammalian calcified tissues using SEM stereophotogrammetry. Scanning Electron Microscopy 1979/2:393–402

    Google Scholar 

  46. Kanehisa J, Heersche JNM (1988) Osteoclastic bone resorption: In vitro analysis of the rate of resorption and migration of individual osteoclasts. Bone 9:73–79

    Article  PubMed  CAS  Google Scholar 

  47. Howell PGT (1978) A theoretical approach to the errors in SEM photogrammetry. Scanning 1:118–124

    Google Scholar 

  48. Boyde A (1970) Practical problems and methods in the threedimensional analysis of scanning electron microscope images. Scanning Electron Microscopy 1970:105–112

    Google Scholar 

  49. Boyde A (1974) A stereo-plotting device for SEM micrographs, and a real time 3-D system for the SEM. Scanning Electron Microsc 1:93–100

    Google Scholar 

  50. Boyde A, Howell PGT, Franc F (1986) Simple SEM stereophotogrammetric method for three-dimensional evaluation of features on flat substrates. J Microsc 143:257–264

    PubMed  CAS  Google Scholar 

  51. Howell PGT, Boyde A, Ross HF (1986) A three axis stereocomparator for scanning electron microscopic photogrammetry-RS3. Scanning 8:182–186

    CAS  Google Scholar 

  52. Boyde A (1986) Stereological stereometry: simplified approaches to volumetry by SEM. Scanning 8:240–249

    CAS  Google Scholar 

  53. Boyde A (1974) Real-time stereo TV speed scanning electron microscopy. Beitr elktronenmikroskop Direktabb Oberfl 7:221–230

    Google Scholar 

  54. Boyde A (1975) Measurement of specimen height difference and beam tilt angle in anaglyph real time stereo TV SEM systems. Scanning Electron Microsc 1975:189–198

    Google Scholar 

  55. Shimizu H, Sakamoto S, Sakamoto M (1989) Matrix collagen of devitalized bone is resistant to osteoclastic bone resorption. Conn Tissue Res 20:169–175

    CAS  Google Scholar 

  56. Shimizu H, Sakamoto M, Sakamoto S (1990) Bone resorption by isolated osteoclasts in living versus devitalized bone: differences in mode and extent and the effects of human recombinant tissue inhibitor of metalloproteinases. J Bone Miner Res 5:411–418

    Article  PubMed  CAS  Google Scholar 

  57. Shimizu H, Sakamoto M, Sakamoto S, Lee DD (1989) The effect of substrate composition and condition on resorption by isolated osteoclasts. Bone Miner 6:261–275

    Article  PubMed  CAS  Google Scholar 

  58. Boyde A (1990) Confocal optical microscopy. In: Duke PJ, Michette AG (eds) Modern microscopies: techniques and applications. Plenum, New York, pp 185–204

    Google Scholar 

  59. Boyde A (1985) The tandem scanning reflected light microscope; part 2: pre-micro '84 applications at UCL. Proc Roy Microsc Soc 20:131–139

    Google Scholar 

  60. Boyde A, Dillon CE, Jones SJ (1990) Measurement of osteoclastic resorption pits with a tandem scanning microscope. J Microsc 158:261–265

    PubMed  CAS  Google Scholar 

  61. Jones SJ, Boyde A, Taylor ML, Maconnachie E (in press) Resorption of both inorganic and organic components of dentine and bone by osteoclasts in vitro. Bone

  62. Boyde A, Maconnachie E, Taylor ML, Jones SJ (in press) Does the rate of organic matrix degradation in in vitro resorption relate to collagen orientation? Bone

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Authors and Affiliations

  1. Department of Anatomy and Development Biology, University of College London, WC1E 6BT, London, England

    Alan Boyde & Sheila J. Jones

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  1. Alan Boyde
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Boyde, A., Jones, S.J. Pitfalls in pit measurement. Calcif Tissue Int 49, 65–70 (1991). https://doi.org/10.1007/BF02565123

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